The Cytomegalovirus (CMV) BIO-PROBE® labeled probe is prepared by nick translation of cloned fragments of the CMV Towne strain. The fragments include a total of 30-31 kb of DNA, approximately 20% of the CMV genome. The probe is specific for cytomegalovirus DNA. It does not hybridize to DNAs of other herpesviruses (i.e., herpes simplex virus or Epstein-Barr virus) but it has been found to hybridize to the DNA of CMV clinical isolates. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).
Product Details
| Concentration: | 20µg/ml |
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| Formulation: | Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA. |
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| Applications: | Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
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| Application Notes: | The Cytomegalovirus (CMV) BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100ng/ml. The probe can also be used for in situ hybridization applications at concentrations of 0.5 to 1.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for one year after receipt when stored as recommended. |
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| Technical Info/Product Notes: | BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Detection of Epstein -Barr genome in the lymph nodes of Hodgkin's disease: C. Bellas, et al.; Postgrad. Med. J.
69, 916 (1993),
Application(s): ISH used with CMV BioProbe as control to study lymp node tissue,
Abstract;
Full Text
Early and Rapid Diagnosis of CMV Infection by Nonradioactive in situ Hybridization in Pediatric Kidney Transplant Recipients: L. Murer, et al.; Nephron
60, 25 (1992),
Application(s): ISH used to diagnose kidney cellular samples,
Abstract;
In situ demonstration of Epstein-Barr virus in intravenous drug abusers with generalized lymphadenopathy: F. Mampaso, et al.; Postgrad. Med. J.
68, 739 (1992),
Application(s): ISH used with CMV BioProbe as control to study lymp node tissue,
Abstract;
Full Text
Non-Hodgkin's lymphoma after prolonged remission of Hodgkin's disease in an HIV-infected patient: C. Montalban, et al.; Ann. Oncol.
8, 585 (1991),
Abstract;
Cytomegalovirus and human herpesvirus-6 in sudden infant death syndrome: an in situ hybridization study: A. Coumbe, et al.; Pediatr. Pathol.
10, 483 (1990),
Abstract;
Demonstration of cytomegalovirus retinitis by in situ DNA hybridization: D. Henderley, et al.; Retina
8, 177 (1988),
Application(s): ISH of retinal tissue,
Abstract;
Direct in situ hybridization for rapid detection of cytomegalovirus in bronchoalveolar lavage: L.H. Hilborne, et al.; Am. J. Clin. Pathol.
87, 766 (1987),
Abstract;
In situ hybridization analysis of cytomegalovirus lytic infection in Kaposi's sarcoma associated with AIDS. A study of 14 autopsy cases: H. Hashimoto, et al.; Virchows Arch. A Pathol. Anat. Histopathol.
411, 441 (1987),
Application(s): CMV assayed with ISH for paraffin embedded samples,
Abstract;
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol.
18, 535 (1987),
Abstract;
Viral Diagnosis by in situ Hybridization: Description of a Rapid Simplified Colorimetric Method: E. Unger, et al.; Am. J. Surg. Pathol.
10, 1 (1986),
Application(s): Colorimetric in situ DNA hybridization to diagnose viral infections,
Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology
126, 32 (1983),
Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS
78, 6633 (1981),
Abstract;
