The Adenovirus BIO-PROBE® labeled probe is prepared by nick translation of cloned fragments of the adenovirus 5 genome. The fragments constitute a total of 20 kb of DNA, approximately 56% of the genome. The probe is specific for adenovirus and, in addition to hybridization to adenovirus 5 sequences, the probe will cross hybridize with adenovirus types 4, 7, 11, 20, 40 and 41 due to their considerable sequence homology. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).
Product Details
Concentration:
20µg/ml
Formulation:
Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.
Applications:
Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
Application Notes:
The Adenovirus BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100ng/ml. The probe can also be used for in situ hybridization applications at concentrations of 0.5 to 1.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes. Complete kits and protocols are available for in situ hybridization and detection procedures (compatible with Enzo’s POLYVIEW® PLUS and SAVIEW® PLUS detection systems for IHC).
Shipping:
Shipped on Dry Ice
Long Term Storage:
-20°C
Use/Stability:
Stable for one year after receipt when stored as recommended.
Technical Info/Product Notes:
BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
Regulatory Status:
RUO - Research Use Only
Product Literature References
An attenuated adenovirus, ONYX-015, as mouthwash therapy for premalignant oral dysplasia: C.M. Rudin, et al.; J. Clin. Oncol. 21, 4546 (2003), Abstract; Full Text
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol. 18, 535 (1987), Abstract;
Detection of adenovirus in nasopharyngeal specimens by radioactive and nonradioactive DNA probes: T. Hyypia; J. Clin. Microbiol. 21, 730 (1985), Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;
