PATHO-GENE® AP-NBT/BCIP HPV in situ typing assay (types 6/11, 16/18 and 31/33/51) - ENZ-32895



ENZ-32895	10 assays	1,750.00 USD
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The PATHO-GENE^® human papillomavirus (HPV) in situ typing assay for tissue sections provides reagents and materials for (a) preparation and pretreatment and (b) hybridization/detection and typing of HPV DNA (using alkaline phosphatase-NBT/BCIP for signal generation) in 10 tissue biopsy specimens. This product is for research use only and is not to be used for diagnostic procedures.

The PATHO-GENE^® human papillomavirus (HPV) in situ typing assay for tissue sections employs separate mixtures of HPV-specific probes to identify HPV types 6/11, 16/18, or 31/33/51. HPV types 6 and 11 are primarily associated with benign lesions presenting as condylomas and mild dysplasia. HPV types 16 and 18 are more commonly associated with cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIS). HPV types 31, 33 or 51 can be associated with either condyloma or CIN and CIS, presumably dependent upon other, as yet undefined, contributing factors or agents. The PATHO-GENE^® human papillomavirus (HPV) in situ typing assay for tissue sections can be used to detect and identify HPV DNA in tissue sections under conditions that preserve cellular morphology.

Product Details

Applications:	Colorimetric detection

Shipping:	Blue Ice

Long Term Storage:	+4°C

Contents:	HPV 6/11 Probe Mix, 0.5 ml (pink) Mixture of biotin-labeled HPV 6 and HPV 11-specific probes in buffered formamide and hybridization enhancers HPV 16/18 Probe Mix, 0.5 ml (purple) Mixture of biotin-labeled HPV 16 and HPV 18-specific probes in buffered formamide and hybridization enhancers HPV 31/33/51 Probe Mix, 0.5 ml (green) Mixture of biotin-labeled HPV 31, HPV 33 and HPV 51-specific probes in buffered formamide and hybridization enhancers Hybridization Wash Reagent, 15 ml (white) Buffered formamide AP Detection Reagent, 15 ml (orange) Streptavidin-biotinylated alkaline phosphatase in buffered saline with stabilizers and preservative NBT Reagent, 0.4 ml (amber) Nitroblue tetrazolium in solvent BCIP Reagent, 0.4 ml (amber) Bromochloroindolyl phosphate in solvent AP Reaction Buffer, 2 x 10 ml (gray) Buffered saline and alkaline phosphatase enhancers Counterstain Concentrate, 30 ml Eosin Y (certified) in alcohol Proteinase K, 5 mg Lyophilized powder SIGNASURE^®Wash Buffer Salts, 4 packets Each packet yields 1 liter of buffer Pretreated Specimen Slides, 20 slides10 single-well plus 10 double-well slides for mounting formalin-fixed paraffin-embedded tissue specimens

Technical Info/Product Notes:	This product or the use of this product is covered by one or more Enzo patents, including, but not limited to, the following: U.S. Patent No. 4,994,373 and Canadian Patent No. 1,309,672; and patents pending.

Regulatory Status:	RUO - Research Use Only

Product Literature References

The utility of p16 immunostaining in fine needle aspiration in p16 positive head and neck squamous cell carcinoma: B. Xu, et al.; Hum. Pathol. 54, 193 (2016), Application(s): CISH using probes against high-risk HPV types, Abstract;

Enhanced expression of PD L1 in cervical intraepithelial neoplasia and cervical cancers: L. Mezache, et al.; Mod. Pathol. 28, 1594 (2015), Application(s): Determination of HPV in human tissue, Abstract;

Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection: G.J. Nuovo, et al.; Diagn. Mol. Pathol. 19, 135 (2010), Abstract; Full Text

In situ determination of T-cell receptor beta expression patterns: G.J. Nuovo, et al.; J. Histochem. Cytochem. 49, 139 (2001), Abstract;

Buschke-Loewenstein tumour infiltrating pelvic organs: A. Grassegger, et al.; Br. J. Dermatol. 130, 221 (1994), Application(s): in situ hybridization, Abstract;

Human papillomavirus detection in genital lesions by in situ hybridization and ultrastructural observations: L.H. Oliveira, et al.; Mem. Inst. Oswaldo Cruz. 89, 195 (1994), Application(s): in situ hybridization, Abstract; Full Text

Histological diagnosis of human papillomavirus (HPV)-related dysplasia: quality control by in situ hybridization (ISH) analysis: L. Cadorin, et al.; Eur. J. Gynaecol. Oncol. 13, 427 (1992), Application(s): in situ hybridization, Abstract;

In situ hybridization for the detection of human papillomavirus (HPV) in gynaecological biopsies. A study of two commercial kits: I. Zehbe, et al.; Anticancer Res. 12, 1383 (1992), Application(s): in situ hybridization, Abstract;

Laryngeal papillomatosis with human papillomavirus DNA contracted by a laser surgeon: P. Hallmo & O. Naess; Eur. Arch. Otorhinolaryngol. 248, 425 (1991), Application(s): in situ hybridization, Abstract; Full Text

Detection of human papillomavirus in nongenital Bowen's disease by in situ DNA hybridization: A.H. Kettler, et al.; Arch. Dermatol. 126, 777 (1990), Application(s): in situ hybridization, Abstract;

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PATHO-GENE^® AP-NBT/BCIP HPV in situ typing assay (types 6/11, 16/18 and 31/33/51)

Product Details

Product Literature References

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In situ hybridization wash reagent

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