The PATHO-GENE® HPV type 16/18 probe is a mixture of biotin-labeled HPV 16 and HPV 18-specific probes in buffered formamide and hybridization enhancers.
Product Details
Application Notes:
The ENZO PATHO-GENE® HPV type 16/18 probe employs a mixture of the HPV 16 and HPV 18-specific probes that detect HPV types that have been associated with cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIS). The probe mixture is denatured and hybridized to the tissue section fixed and pretreated on the microscope slide. Specific hybridization between the HPV DNA probe and DNA in the specimen is determined by the detection of biotin.
Shipping:
Blue Ice
Long Term Storage:
+4°C
Use/Stability:
Stable for at least one year after receipt when stored as recommended.
Technical Info/Product Notes:
HPV probe can be optimized for polymer detection system using diluent buffer, In situ hybridization buffer for HPV probes (Ready-to Use) (Prod. No. ENZ-33905).
This product or the use of this product is covered by one or more Enzo patents, including, but not limited to the following: U.S. Patent No. 4,994,373; Canadian Patent No. 1,309,672; and patents pending.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Clinical outcomes in elderly patients with human papillomavirus–positive squamous cell carcinoma of the oropharynx treated with definitive chemoradiation therapy: S. Hanasoge, et al.; Head Neck 38, 846 (2016), Abstract;
Polyomavirus large T antigen is prevalent in urothelial carcinoma post-kidney transplant: L. Yan, et al.; Hum. Pathol. 48, 122 (2016), Abstract;
β-catenin expression in oropharyngeal squamous cell carcinomas: comparison and correlation with p16 and human papillomavirus in situ hybridization: J. Nwanze, et al.; Acta Cytol. 59, 479 (2015), Abstract;
Automated In Situ Hybridization For Human Papilloma Virus: C. Cohen, et al.; Appl. Immunohistochem. Mol. Morphol. 22, 619 (2014), Abstract;
Mutation and transcriptional profiling of formalin-fixed paraffin embedded specimens as companion methods to immunohistochemistry for determining therapeutic targets in oropharyngeal squamous cell carcinoma (OPSCC): a pilot of proof of principle: N.F. Saba, et al.; Head Neck Pathol. 9, 223 (2014), Abstract;
Automated and manual human papilloma virus in situ hybridization and p16 immunohistochemistry: comparison in metastatic oropharyngeal carcinoma: N. Fatima, et al.; Acta Cytol. 57, 633 (2013), Abstract;
Prognostic value of HPV18 DNA viral load in patients with early-stage neuroendocrine carcinoma of the uterine cervix: S. Siriaunkgul, et al.; Asian Pac. J. Cancer Prev. 13, 3281 (2012), Abstract;
Detection of integrated human papillomavirus by human papillomavirus types 16 and 18 in situ hybridization: a valuable diagnostic tool in diagnosing cervical carcinoma?: R.J. van Golde, et al.; Am. J. Obstet. Gynecol. 199, e8 (2008), Abstract;
p16 expression in relation to human papillomavirus in liquid-based cervical smears: B. Samama, et al.; Gynecol. Oncol. 109, 285 (2008), Abstract;
Time course of juvenile onset recurrent respiratory papillomatosis caused by human papillomavirus: M. Hawkes, et al.; Pediatr. Infect. Dis. J. 27, 149 (2008), Abstract;
Detection of specific human papillomavirus types in paraffin-embedded sections of cervical carcinomas: J.T. Bryan, et al.; J. Med. Virol. 78, 117 (2006), Abstract;
p16 expression in relation to human papillomavirus in anogenital lesions: B. Samama, et al.; Hum. Pathol. 37, 513 (2006), Abstract;
Detection of oncogenic virus genomes and gene products in lung carcinoma: L. Brouchet, et al.; Br. J. Cancer 92, 743 (2005), Abstract;
Comparison of real-time PCR signal-amplified in situ hybridization and conventional PCR for detection and quantification of human papillomavirus in archival cervical cancer tissue: K. Biedermann, et al.; J. Clin. Microbiol. 42, 3758 (2004), Abstract;
A cardiac allograft recipient with Bowen's disease on a finger and concurrent perianal bowenoid papulosis: M. Ibe, et al.; J. Dermatol. 30, 389 (2003), Abstract;
DNA copy number changes in cervical adenocarcinoma: A. Dellas, et al.; Clin. Cancer Res. 9, 2985 (2003), Abstract;
Evidence for at least three alternative mechanisms targeting the p16INK4A/cyclin D/Rb pathway in penile carcinoma, one of which is mediated by high-risk human papillomavirus: E. Ferreux, et al.; J. Pathol. 201, 109 (2003), Abstract;
Morphologic and biologic studies on ten cases of verrucous carcinoma of the vulva supporting the theory of a discrete clinico-pathologic entity: M. Gualco, et al.; Int. J. Gynecol. Cancer 13, 317 (2003), Abstract;
Pearly penile papules: still no reason for uneasiness: C.J. Hogewoning, et al.; J. Am. Acad. Dermatol. 49, 50 (2003), Abstract;
Detection of kappa- and lambda-expressing cells in the endometrium by in situ hybridization: E. Euscher, et al.; Int. J. Gynecol. Pathol. 21, 383 (2002), Abstract;
HPV DNA detection by in situ hybridization with catalyzed signal amplification on thin-layer cervical smears: B. Samama, et al.; J. Histochem. Cytochem. 50, 1417 (2002), Abstract;
Penile lesions and human papillomavirus in male sexual partners of women with cervical intraepithelial neoplasia: M.C. Bleeker, et al.; J. Am. Acad. Dermatol. 47, 351 (2002), Abstract;
Primary inverted papilloma of the middle ear and mastoid: C. de Filippis, et al.; Otol. Neurotol. 23, 555 (2002), Abstract;
Human papillomaviruses 6/11, 16/18 and 31/33/51 are not associated with squamous cell carcinoma of the urinary bladder: P.J. Westenend, et al.; BJU Int. 88, 198 (2001), Abstract;
Significance of human papillomavirus in sinonasal papillomas: M. Kraft, et al.; J. Laryngol. Otol. 115, 709 (2001), Abstract;
Squamous papilloma of the esophagus: long-term follow up: S. Mosca, et al.; J. Gastroenterol. Hepatol. 16, 857 (2001), Abstract;
MIB-1 immunostaining is a beneficial adjunct test for accurate diagnosis of vulvar condyloma acuminatum: E.C. Pirog, et al.; Am. J. Surg. Pathol. 24, 1393 (2000), Abstract;
Squamous papilloma of the urinary tract is unrelated to condyloma acuminata: L. Cheng, et al.; Cancer 88, 1679 (2000), Abstract;
Adenocarcinoma in situ of the uterine cervix: clinicopathological study of nine cases with detection of human papillomavirus DNA by in situ hybridization and polymerase chain reaction: M. Alejo, et al.; Int. J. Gynecol. Pathol. 12, 219 (1993), Application(s): in situ hybridization, Abstract;
Correlation of human papillomavirus DNA detection in biopsies of cervical lesions and the corresponding cervical swabs with the same method of in situ hybridization: K. U. Petry, et al.; Acta Virol. 37, 251 (1993), Application(s): in situ hybridization, Abstract;
Detection of human papillomavirus 16 and 18 DNA in epithelial lesions of the lower genital tract by in situ hybridization and polymerase chain reaction: cervical scrapes are not substitutes for biopsies: N. Margall, et al.; J. Clin. Microbiol. 31, 924 (1993), Application(s): in situ hybridization, Abstract; Full Text
Enhanced chemiluminescence: a high-sensitivity detection system for in situ hybridization and immunohistochemistry: P. Lorimier, et al.; J. Histochem. Cytochem. 41, 1591 (1993), Application(s): in situ hybridization, Abstract; Full Text
Use of Probemix and OmniProbe biotinylated cDNA probes for detecting HPV infection in biopsy specimens from the genital tract: I. Zehbe, et al.; J. Clin. Pathol. 46, 437 (1993), Application(s): in situ hybridization, Abstract; Full Text
Correlation of the expression of Epstein-Barr virus latent membrane protein and in situ hybridization with biotinylated BamHI-W probes in Hodgkin's disease: G. Delsol, et al.; Am. J. Pathol. 140, 247 (1992), Application(s): negative controls, Abstract; Full Text
Human papillomavirus infection and anal carcinoma. Retrospective analysis by in situ hybridization and the polymerase chain reaction: S.R. Zaki, et al.; Am. J. Pathol. 140, 1345 (1992), Application(s): in situ hybridization, Abstract; Full Text
Localization of HPV-16 DNA sequence in CaSki cells by electron microscopic hybridocytochemistry: C.T. Lin, et al.; J. Histochem. Cytochem. 40, 467 (1992), Application(s): Southern Blot, Abstract; Full Text
Enhanced chemiluminescence for tissue antigen and cellular viral DNA detection: E. Hawkins, et al.; J. Histochem. Cytochem. 38, 415 (1990), Application(s): in situ hybridization, Abstract; Full Text
Sensitivity of in situ detection with biotinylated probes of human papilloma virus type 16 DNA in frozen tissue sections of squamous cell carcinomas of the cervix: J.M. Walboomers, et al.; Am. J. Pathol. 131, 587 (1988), Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;
