Product Details
| Clone: | HMB45 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Human axillary lymph node tissue containing pigmented melanoma metastases. |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human
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| Specificity: | Recognizes a human melanoma-specific antigen present in melanocytic cells and junctional nevi. |
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| Crossreactivity: | Does not cross-react with normal melanocytes, intradermal nevus cells or other neoplasms including sarcomas, carcinomas and glial tumors. |
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| Applications: | IHC (PS)
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections; this antibody can be used for staining of paraffin-embedded tissue sections fixed in neutral buffered formalin and is compatible with most commonly used histological fixatives. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually. |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Liquid. Ready-to-use solution in PBS containing 0.1% sodium azide and 1% BSA. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1. Immunohistochemistry analysis of human melanoma skin tissue using Melanoma marker (human) mAb (HMB45) (Ready-To-Use), (Prod. No. ENZ-30930). Antibody is applied as is.
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Product Literature References
Sclerosing Epithelioid Fibrosarcoma of the Kidney: Clinicopathologic and Molecular Study of a Rare Neoplasm at a Novel Location: C.H. Ohlmann, et al.; Ann. Diagn. Pathol.
19, 221 (2015),
Application(s): Immunohistochemistry,
Abstract;
Comparison of HMB-45 monoclonal antibody and S-100 protein in the immunohistochemical diagnosis of melanoma: N.G. Ordonez, et al.; Am. J. Clin. Pathol.
90, 385 (1988),
Abstract;
Immunohistochemical diagnosis of sinonasal melanoma, carcinoma, and neuroblastoma with monoclonal antibodies HMB-45 and anti-synaptophysin: M.R. Wick, et al.; Arch. Pathol. Lab. Med.
112, 616 (1988),
Abstract;
Melanoma cell heterogeneity. A study of two monoclonal antibodies compared with S-100 protein in paraffin sections: P.H. Duray, et al.; Cancer
61, 2460 (1988),
Abstract;
Recognition of malignant melanoma by monoclonal antibody HMB-45. An immunohistochemical study of 200 paraffin-embedded cutaneous tumors: M.R. Wick, et al.; J. Cutan. Pathol.
15, 201 (1988),
Abstract;
Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes: A.M. Gown, et al.; Am. J. Pathol.
123, 195 (1986),
Abstract;
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