Product Details
| Alternative Name: | HMWCK, High molecular weight cytokeratin, Keratin-903 |
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| Clone: | 34βE12 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Keratin solubilized from human stratum corneum. |
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| UniProt ID: | P04264 (CK1), P13647 (CK5), P13645 (CK10), P02533 (CK14) |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human
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| Specificity: | 34βE12 is specific for “high molecular weight” cytokeratins 1, 5, 10 and 14, corresponding to molecular weights 68, 58, 56.5 and 50 kDa, respectively, which are characteristically found in “complex” epithelium. The antibody reacts with all squamous and ductal epithelium as well as carcinomas such as squamous cell carcinoma and adenocarcinoma of ductal origin (e.g., breast, pancreas) but does not react with sarcoma, melanoma or lymphoma. This antibody reacts with benign small-acinar lesions of the prostate and has been shown to be useful as an adjunct in the identification of prostate carcinoma. |
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| Applications: | IHC (PS), WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections; for optimal results, fixation in alcohol-based fixatives, such as methacarn, is recommended. Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained. For optimal staining of prostate tissue higher concentrations of protease and longer incubation times are recommended, e.g., 0.5% trypsin at 37°C for 2 to 4 hours. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Liquid. Ready-to-use solution in PBS containing 0.1% sodium azide and 1% BSA. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Regulatory Status: | RUO - Research Use Only |
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Prostate tissue assayed with Cytokeratin 1/5/10/14 (human) monoclonal antibody (34βE12) (ENZ-30903). Developed with POLYVIEW® PLUS HRP (anti-mouse) reagent (ENZ-ACC104) and HIGHDEF® DAB Chromogen/Substrate Set (ENZ-ACC105).
Prostate tissue assayed with Cytokeratin 1/5/10/14 (human) monoclonal antibody (34βE12) (ENZ-30903). Developed with POLYVIEW® PLUS HRP (anti-mouse) reagent (ENZ-ACC104) and HIGHDEF® DAB Chromogen/Substrate Set (ENZ-ACC105).
Immunohistochemistry analysis of human skin tissue using Cytokeratin 1/5/10/14 (human), mAb (34βE12), (Prod. No. ENZ-30903).
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Cytoplasmic PTEN protein loss distinguishes intraductal carcinoma of the prostate from high-grade prostatic intraepithelial neoplasia: T.L. Lotan, et al.; Mod. Pathol.
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Immunohistochemical markers for the differential diagnosis of nephrogenic adenomas: W. Quinones, et al.; Ann. Diagn. Pathol.
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Prostate cancer cell telomere length variability and stromal cell telomere length as prognostic markers for metastasis and death: C.M. Heaphy, et al.; Cancer Discov.
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Application(s): Immunohistochemistry multiplexed with fluorescent in-situ hybridization using formalin-fixed, paraffin-embedded human prostate carcinoma tissue samples,
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Immunohistochemical Staining with DeltaNp63 Is Useful for Distinguishing the Squamous Cell Component of Adenosquamous Cell Carcinoma of the Lung: H. Uramoto, et al.; Anticancer Res.
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Application(s): IHC using human lung cancer (ADSQ) samples,
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Primary lymphoepithelioma-like carcinoma of the ureter: D.S. Allende, et al.; Ann. Diagn. Pathol.
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Somatic but Not Germline Mutation of the APC Gene in a Case of Cribriform-Morular Variant of Papillary Thyroid Carcinoma: J. Cameselle-Teijeiro, et al.; Am. J. Clin. Pathol.
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Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18: D. Bello, et al.; Carcinogenesis
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Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies: A.M. Gown & A.M. Vogel; J. Cell Biol.
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