Product Details
| Alternative Name: | CK8 |
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| Clone: | 35βH11 |
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| Host: | Mouse |
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| Isotype: | IgM |
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| Immunogen: | Triton/High salt-insoluble material from the human hepatocellular carcinoma cell line Hep3B. |
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| UniProt ID: | P05787 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Human
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| Specificity: | 35βH11 is specific for "low molecular weight" cytokeratin 8 (52.5 kDa) characteristically found in "simple" epithelium (e.g., hepatocytes, pancreatic acinar cells, absorptive cells of the GI tract, etc.), ductal structures (e.g., of liver and breast), but not in squamous epithelium. The antibody stains carcinomas such as ductal carcinoma of the breast, liver and pancreas, adenocarcinoma (ovary, lung, endometrium) but does not react with squamous cell carcinoma, sarcoma, melanoma or lymphoma. When used in conjunction with MAb to Cytokeratin 1/5/10/14 (human) (34βE12) (Prod. No. ENZ-30903), the majority of epithelium and epithelial tumors are labeled. |
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| Applications: | IHC (PS), WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections; for optimal results, fixation in alcohol-based fixatives, such as methacarn, is recommended. Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained. Choose a good quality enzyme-linked anti-mouse IgM antibody for detection)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Liquid. Ready-to-use solution in PBS containing 0.1% sodium azide and 1% BSA. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | +4°C |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human pancreas tissue using Cytokeratin 8 (human), mAb (35βH11) at a dilution of 1:1.
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Product Literature References
Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues: A.M. Gown & A.M. Vogel; Am. J. Pathol.
114, 309 (1984),
Abstract;
Full Text
Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies: A.M. Gown & A.M. Vogel; J. Cell Biol.
95, 414 (1982),
Abstract;
Full Text
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