| Clone: | M36 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Mixture of PCI in complex with PSA (approx. 80%) and PCI cleaved from such a complex (approx. 20%). |
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| UniProt ID: | P05154 (human PCI) |
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| Gene/Protein Identifier: | 5104 (Gene ID) |
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| Species reactivity: | Human
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| Specificity: | BPD-JST-001-38 is specific for APC:PCI complex and cleaved PCI but has little reactivity towards native PCI. The dissociation constants (as determined by standard surface plasmon resonance technique) are: Kd= 4x10e-10 M for PCI in complex with APC and 2x10e-10 M for cleaved PCI. The value of Kd for native PCI is too low to measure, i.e. > 10e-5 M. |
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| Applications: | ELISA, WB
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| Purity Detail: | Protein A-affinity purified. |
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| Formulation: | Liquid. In 0.01 M phosphate buffer, pH 7.4, containing 0.14 M sodium chloride and 15 mM sodium azide. |
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Activated Protein C (APC), a serine proteinase is inhibited by Protein C inhibitor (PCI). PCI belongs to a group of inhibitors sometimes referred to as serpines (serine proteinase inhibitors), and form 1:1 complexes with APC. PCI plasma concentration is approximately 4 µg/ml. The complex formation between APC and PCI proceeds at a slow rate, which is manifested by a long half-life for APC in plasma, wherein t1/2 is about 20 min. However, the rate of complex formation between APC and PCI is increased by heparin. Upon complex formation with APC, the serpin is cleaved in its so-called bait region, whereby a stable intermediate acyl complex is formed. With time, the intermediate acyl complex dissociates, whereby APC is regenerated and a proteolytically modified, i. e. cleaved, inactive serine proteinase inhibitor is formed. |
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| Regulatory Status: | RUO - Research Use Only |
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