| Alternative Name: | Activated protein C and α1-antitrypsin complex |
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| Clone: | PC 7 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Recombinant human activated protein C. |
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| UniProt ID: | P04070 (APC), P01009 (AAT) |
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| Source: | From culture supernatant. |
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| Species reactivity: | Human
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| Specificity: | Specific for a conformation-dependent neoepitope that is expressed in activated protein C upon complex-formation with α1-antitrypsin. No reaction is seen to non-complexed α1-antitrypsin and only very little cross reaction to protein C zymogen. Note that specificity is calcium dependent. |
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| Applications: | ELISA
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| Purity Detail: | Protein A/G-purified. |
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| Formulation: | Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.5M sodium chloride and 15mM sodium azide. |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62kDa proenzyme is activated by thrombin, and the active enzyme cleaves factor Va and VIIIa, thus inhibiting blood coagulation. The molecular weight of the active enzyme is 55kDa. The normal concentration in human plasma is approximately 1-3 ng/mL due to the very fast turnover and the proenzyme concentration of approximately 3 ug/mL. The activated protein C (APC) is inhibited by members of the serine protease inhibitor (serpin) family, of which alpha1-antitrypsin (AAT) and protein C inhibitor (PCI) are the most important. |
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| Regulatory Status: | RUO - Research Use Only |
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