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BML-ZW9345-0005 5 mg 215.00 USD
BML-ZW9345-0050 50 mg 1,693.00 USD
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Replaces Prod. #: ALX-260-086

Fluorogenic substrate for the peptidylglutamyl-peptide hydrolysing (caspase-like) activity of the proteasome which may be stimulated in the presence of Mg2+ ions and rapidly inactivated by N-acetylimidazole.

Product Details

Alternative Name:Z-LLE-AMC, Proteasome substrate II (fluorogenic)
Sequence:Z-LLE-AMC (AMC=7-Amino-4-methylcoumarin)
MW:664.75 Da
Purity:≥95% (HPLC)
Appearance:Lyophilized. Off white powder.
Solubility:Soluble in dimethylformamide.
Shipping:Blue Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only

Product Literature References

The role of proteasome in muscle wasting of experimental arthritis: V.O.N. Teixeira, et al.; Adv. Rheumatol. 63, 14 (2023), Abstract;
Comprehensive Analysis of Proteasomal Complexes in Mouse Brain Regions Detects ENO2 as a Potential Partner of the Proteasome in the Striatum: N. Esfahanian, et al.; Cell. Mol. Neurobiol. 42, 2305 (2022), Abstract;
Innate Variability in Physiological and Omics Aspects of the Beta Thalassemia Trait-Specific Donor Variation Effects: A.T. Anastasiadi, et al.; Front. Physiol. 13, 907444 (2022), Abstract;
Short-term exposure to an obesogenic diet causes dynamic dysregulation of proteasome-mediated protein degradation in the hypothalamus of female rats: T. McFadden, et al.; Nutr. Neurosci. 13, 1 (2022), Abstract;
Supplementation with uric and ascorbic acid protects stored red blood cells through enhancement of non-enzymatic antioxidant activity and metabolic rewiring: V.L. Tzounakas, et al.; Redox Biol. 57, 102477 (2022), Abstract;
The molecular network of the proteasome machinery inhibition response is orchestrated by HSP70, revealing vulnerabilities in cancer cells: M. Oroń, et al.; Cell Rep. 40, 111428 (2022), Abstract;
20S proteasomes secreted by the malaria parasite promote its growth: E. Dekel, et al.; Nat. Commun. 12, 1172 (2021), Abstract; Full Text
A Transgenic Mouse Model of Eccentric Left Ventricular Hypertrophy With Preserved Ejection Fraction Exhibits Alterations in the Autophagy-Lysosomal Pathway: K. Wenzel, et al.; Front. Physiol. 12, 614878 (2021), Abstract;
Amniotic Fluid Proteasome and Immunoproteasome in the Setting of Intra-Amniotic Infection, Inflammation, and Preterm Birth: C.A. Ware, et al.; Reprod Sci. 28, 2562 (2021), Abstract;
Aromatic monophenols from cinnamon bark act as proteasome inhibitors by upregulating ER stress, suppressing FoxM1 expression, and inducing apoptosis in prostate cancer cells: S. Gopalakrishnan, et al.; Phytother. Res. 35, 5781 (2021), Abstract;
Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome: M.M. Barsan, et al.; Sensors 21, 2702 (2021), Abstract; Full Text
Nrf2 activation induces mitophagy and reverses Parkin/Pink1 knock down-mediated neuronal and muscle degeneration phenotypes: S. Gumeni, et al.; Cell Death Dis. 12, 41419 (2021), Abstract;
Red Blood Cell Proteasome in Beta-Thalassemia Trait: Topology of Activity and Networking in Blood Bank Conditions: A.T. Anastasiadi, et al.; Membranes 11, 716 (2021), Abstract;
Targeting AML dependency on VCP-mediated DNA repair through a selective second-generation small molecule inhibitor: B. Roux, et al.; Sci. Transl. Med. 13, eabg1168 (2021), Abstract;
Biological Evaluation and In Silico Study of Benzoic Acid Derivatives from Bjerkandera adusta Targeting Proteostasis Network Modules: K. Georgousaki, et al.; Molecules 25, 666 (2020), Application(s): Proteasome activity in human fibroblasts lysates., Abstract; Full Text
Cell cycle-dependent localization of the proteasome to chromatin: Y. Kito, et al.; Sci. Rep. 10, 5801 (2020), Abstract; Full Text
Gender-specific changes in energy metabolism and protein degradation as major pathways affected in livers of mice treated with ibuprofen: S. Tiwari, et al.; Sci. Rep. 10, 3386 (2020), Abstract; Full Text
Reproductive Potential of Yeast Cells Depends on Overall Action of Interconnected Changes in Central Carbon Metabolism, Cellular Biosynthetic Capacity, and Proteostasis: R. Maslanka & R. Zadrag-Tecza; Int. J. Mol. Sci. 21, 7313 (2020), Abstract; Full Text
Males and Females Differ in the Subcellular and Brain Region Dependent Regulation of Proteasome Activity by CaMKII and Protein Kinase A: R.K. Devulapalli, et al.; Neuroscience 418, 1 (2019), Abstract;
Mass spectrometry-based absolute quantification of 20S proteasome status for controlled ex-vivo expansion of Human Adipose-derived Mesenchymal Stromal/Stem Cells: T. Menneteau, et al.; Mol. Cell. Proteomics 18, 744 (2019), Abstract;
A leptospiral AAA+ chaperone-Ntn peptidase complex, HslUV, contributes to the intracellular survival of Leptospira interrogans in hosts and the transmission of leptospirosis: S.L. Dong, et al.; Emerg. Microbes Infect. 6, e105 (2017), Abstract; Full Text
A lowered 26S proteasome activity correlates with mantle lymphoma cell lines resistance to genotoxic stress: K. Ben Younes, et al.; BMC Cancer 17, 538 (2017), Abstract; Full Text
The Architecture of the Anbu Complex Reflects an Evolutionary Intermediate at the Origin of the Proteasome System: A.C.D. Fuchs, et al.; Structure 25, 834 (2017), Abstract; Full Text
Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases: L. Lauinger, et al.; Nat. Chem. Biol. 13, 709 (2017), Abstract; Full Text
Cardiac proteasome functional insufficiency plays a pathogenic role in diabetic cardiomyopathy: J. Li, et al.; J. Mol. Cell. Cardiol. 102, 53 (2016), Abstract;
Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation: W.H. Choi, et al.; Nat. Commun. 7, 10963 (2016), Application(s): Western blot, Abstract;
Pim1 inhibition as a novel therapeutic strategy for Alzheimer’s disease: R. Velazquez, et al.; Mol. Neurodegener. 11, 52 (2016), Application(s): Proteasome activity, brain homogenate, Abstract; Full Text
The immunoproteasomes are key to regulate myokines and MHC class I expression in idiopathic inflammatory myopathies: S. Bhattarai, et al.; J. Autoimmun. 75, 118 (2016), Application(s): Measurement of CT-L and CL proteasome activity, Abstract;
FV-162 is a novel, orally bioavailable, irreversible proteasome inhibitor with improved pharmacokinetics displaying preclinical efficacy with continuous daily dosing: Z. Wang, et al.; Cell Death Dis. 6, e1815 (2015), Application(s): Proteasome activity assays, Abstract; Full Text
Hexapeptide-11 is a novel modulator of the proteostasis network in human diploid fibroblasts: A.D. Sklirou, et al.; Redox Biol. 5, 205 (2015), Application(s): Fluorescent detection, Abstract;
The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget 5, 6252 (2014), Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay, Abstract; Full Text
Marchantin M: a novel inhibitor of proteasome induces autophagic cell death in prostate cancer cells: H. Jiang, et al.; Cell Death Dis. 4, e761 (2013), Application(s): Fluorogenic substrate for kinetics assay of 20S proteasome, Abstract; Full Text
STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy: J.V. Ferreira, et al.; Autophagy 9, 1349 (2013), Abstract;

General Literature References

Purification and characterization of a Z-Leu-Leu-Leu-MCA degrading protease expected to regulate neurite formation: a novel catalytic activity in proteasome.: S. Tsubuki et al.; Biochem. Biophys. Res. Commun. 196, 1195 (1993), Abstract;
Enzymatic changes of the bovine pituitary multicatalytic proteinase complex, induced by magnesium ions.: M.E. Pereira et al.; Arch. Biochem. Biophys. 294, 1 (1992), Abstract;
Chemical modification of the bovine pituitary multicatalytic proteinase complex by N-acetylimidazole. Reversible activation of casein hydrolysis.: B. Yu et al.; J. Biol. Chem. 266, 17396 (1991), Abstract;
Regulation of the peptidylglutamyl-peptide hydrolyzing activity of the pituitary multicatalytic proteinase complex.: M. Orlowski et al.; Biochemistry 30, 5999 (1991), Abstract;

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