| MW: | 113 kDa |
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| Source: | Produced in E. coli. Human ISG15 E1 is fused at the C-terminus to a His-tag. |
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| UniProt ID: | P41226 |
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| Formulation: | Liquid. In 50mM TRIS-Cl, pH 7.5, 50mM NaCl, 1mM DTT. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Purity Detail: | Purified by Ni-affinity chromatography. |
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| Application Notes: | Uses:
Activation of ISG15 for ISGylation of specific proteins in presence of dedicated E2 conjugating/E3 ligase enzymes.
ISGylation pathway studies. Test proteins for ISG15 E3 ligase activity.
Test proteins as potential targets for ISG15 modification. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | The interferon (IFN)-α/β-induced ISG15 E1 activating enzyme, also known as known as Ube1L, is a single-subunit, ATP dependent enzyme that facilitates conjugation of ISG15 to cellular proteins via the ISGylation pathway. |
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| Regulatory Status: | RUO - Research Use Only |
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