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SUMO-3 (human), (recombinant) (His-tag)

BML-UW9215-0500 500 µg 281.00 USD
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Mature protein for functional studies.

Product Details

Alternative Name:Sentrin-3, Small ubiquitin-related modifier 3, SUMO-2
MW:15.5 kDa
Source:Produced in E. coli. Human SUMO-3 is fused to an N-terminal His-tag.
UniProt ID:P55854
Formulation:Liquid. In 20mM HEPES, pH 8.0, containing 50mM sodium chloride and 1mM dithiothreitol.
Purity:≥95% (SDS-PAGE)
Quality Control:Ability to SUMOlyate RanGAP substrate (Prod. No. BML-UW9755) in vitro.
Application Notes:Uses:
1. SUMO-3 modification of specific proteins in vitro.
2. Demonstrate novel proteins are potential targets for SUMOylation under in vitro conditions.
3. Generate substrates for deSUMOylating enzymes.
Shipping:Dry Ice
Long Term Storage:-80°C
Scientific Background:Conjugation of mature SUMO proteins to specific lysine residues on target proteins via isopeptide bonds, is involved in a range of processes including nuclear protein targeting, formation of sub-nuclear complexes, regulation of transcriptional activities, and control of protein stability.
Regulatory Status:RUO - Research Use Only
SUMO-3 (human), (recombinant) (His-tag) Western blot
Western Blot analysis: Lane 1: MW marker, Lane 2: SUMO-3 probed with SUMO-2/3 pAb.
SUMO-3 (human), (recombinant) (His-tag) SDS-PAGE
SDS-PAGE analysis: Lane 1: MWM, Lane 2: 1μg SUMO-3 recombinant protein.
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SUMO-3 (human), (recombinant) (His-tag) Western blot SUMO-3 (human), (recombinant) (His-tag) SDS-PAGE

Product Literature References

Resolving the zinc binding capacity of honey bee vitellogenin and locating its putative binding sites: V. Leipart, et al.; Insect Mol. Biol. 31, 810 (2022), Abstract;

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