Biotinylated ubiquitin (prepared by the action of biotinamidocaproic acid N-hydroxysuccinimide ester on ubiquitin) is useful for studying ubiquitinylation in vitro, where it represents an alternative to radio-iodinated ubiquitin. This product contains a number of multiply biotinylated ubiquitin species, which has proven itself to be a reagent of considerable utility particularly for carrying out E1/E2-mediated thiolester assays.
Product Details
| Source: | Produced in E. coli. |
| |
| UniProt ID: | P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52) |
| |
| Formulation: | Lyophilized from Acetonitrile:water (1:1). |
| |
| Purity Detail: | Mass spectral analysis shows presence of [biotinyl]1-5-ubiquitin with [biotinyl]2-4-ubiquitin being the predominate species. |
| |
| Appearance: | White solid. |
| |
| Application Notes: | Biotinylated ubiquitin is activated by E1 and is a competent substrate for E2 and E3 enzymes. The biotinyl moiety also provides a potential route to affinity purification of labeled species. |
| |
| Solubility: | In order to ensure full solubility it is recommended that the material be dissolved in 5µL DMSO prior to dilution with the buffer of choice to the desired working concentration. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
CD95/Fas suppresses NF-kB activation through recruitment of KPC2 in a CD95L/FasL-independent mechanism: J.P. Guégan, et al.; iScience
24, 103538 (2021),
Abstract;
BRCA1 through Its E3 Ligase Activity Regulates the Transcription Factor Oct1 and Carbohydrate Metabolism: K. Vazquez-Arreguin, et al.; Mol. Cancer Res.
16, 439 (2018),
Abstract;
