Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Ubiquitin, (biotinylated)

BML-UW8705-0100 100 µg 210.00 USD
Do you need bulk/larger quantities?
Biotinylated ubiquitin (prepared by the action of biotinamidocaproic acid N-hydroxysuccinimide ester on ubiquitin) is useful for studying ubiquitinylation in vitro, where it represents an alternative to radio-iodinated ubiquitin. This product contains a number of multiply biotinylated ubiquitin species, which has proven itself to be a reagent of considerable utility particularly for carrying out E1/E2-mediated thiolester assays.

Product Details

Source:Produced in E. coli.
UniProt ID:P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
Formulation:Lyophilized from Acetonitrile:water (1:1).
Purity Detail:Mass spectral analysis shows presence of [biotinyl]1-5-ubiquitin with [biotinyl]2-4-ubiquitin being the predominate species.
Appearance:White solid.
Application Notes:Biotinylated ubiquitin is activated by E1 and is a competent substrate for E2 and E3 enzymes. The biotinyl moiety also provides a potential route to affinity purification of labeled species.
Solubility:In order to ensure full solubility it is recommended that the material be dissolved in 5µL DMSO prior to dilution with the buffer of choice to the desired working concentration.
Shipping:Blue Ice
Long Term Storage:-20°C
Handling:Avoid freeze/thaw cycles.
Regulatory Status:RUO - Research Use Only

Product Literature References

CD95/Fas suppresses NF-kB activation through recruitment of KPC2 in a CD95L/FasL-independent mechanism: J.P. Guégan, et al.; iScience 24, 103538 (2021), Abstract;
BRCA1 through Its E3 Ligase Activity Regulates the Transcription Factor Oct1 and Carbohydrate Metabolism: K. Vazquez-Arreguin, et al.; Mol. Cancer Res. 16, 439 (2018), Abstract;

Related Literature

Stem Cells
Stem Cells
Download as PDF

Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

All new literature pieces