Product Specification
| Alternative Name: | NEDD8-conjugating enzyme UBE2F |
| |
| MW: | ~23.2kDa |
| |
| Source: | Produced in E. coli. |
| |
| UniProt ID: | Q969M7 |
| |
| Formulation: | Liquid. In PBS. |
| |
| Purity: | ≥95% (SDS-PAGE) |
| |
| Purity Detail: | Purified by Ni-affinity chromatography. |
| |
| Application Notes: | For use in NEDD8 conjugation studies. |
| |
| Shipping: | Shipped on Dry Ice |
| |
| Short Term Storage: | -80°C |
| |
| Long Term Storage: | -80°C |
| |
| Use/Stability: | Stable for at least 1 year after receipt when stored, as supplied, at -80°C. |
| |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
| |
| Scientific Background: | Cullin-RING ligases (CRLs) comprise the largest subfamily of E3 ubiquitin ligases. In humans, six cullins (CUL1, -2, -3, -4A, -4B, and -5), two RBX-family RING proteins (RBX1 and -2), and hundreds of substrate receptors assemble into distinct CRLs that mediate ubiquitination of thousands of targets to regulate a vast array of cellular processes. CRL function is regulated by attachment of the ubiquitin-like protein (UBL) NEDD8 to a conserved lysine in a cullin’s C-terminal domain. NEDD8 both enhances intrinsic CRL ubiquitination activity and prevents CRLs binding to the inhibitor CAND1. The NEDDylation cascade employs one of two NEDD8 E2 conjugating enzymes, UbcH12 (aka Ube2M, BML-UW9145) and Ube2F, to facilitate this modification. Ube2F has been shown to be functionally distinct from UbcH12, interacting with RBX2 to target different cullins for NEDDylation. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Figure: SDS-PAGE of His6-Ube2F (Prod. No. BML-UW0940) (coomassie staining).Lane 1: MW markers. Lane 2: His6-Ube2F (3μg).
Please mouse over
General Literature References
E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification: D.T. Huang, et al.; Mol. Cell
33, 483 (2009),
Abstract;
Function and regulation of cullin-RING ubiquitin ligases: M.D. Petroski & R.J. Deshaies; Nat. Rev. Mol. Cell Biol.
6, 9 (2005),
Abstract;
A hitchhiker's guide to the cullin ubiquitin ligases: SCF and its kin: A.R. Willems, et al.; Biochim. Biophys. Acta
1695, 133 (2004),
Abstract;
Nedd8 on cullin: building an expressway to protein destruction: Z.Q. Pan, et al.; Oncogene
23, 1985 (2004),
Abstract;
NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases: J. Liu, et al.; Mol. Cell
10, 1511 (2002),
Abstract;
A Nedd8 conjugation pathway is essential for proteolytic targeting of p27Kip1 by ubiquitination: V.N. Podust, et al.; PNAS
97, 4579 (2000),
Abstract;
