Product Details
| MW: | Range |
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| Source: | Synthetic. Ubiquitin was polymerized through Lys48 using E1, UbcH5A, and the C-terminal domain of a specific HECT E3. |
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| UniProt ID: | P0CG47 (UBB), P0CG48 (UBC) |
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| Formulation: | Lyophilized. |
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| Purity Detail: | Purified by subtractive anion exchange chromatography. |
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| Application Notes: | For use in deubiquitinylating enzyme assays and polyubiquitin binding studies.
Suggested uses:
1. Deubiquitinylating enzyme substrates (general/linkage specific).
2. Investigation of polyubiquitin chain recognition by and interaction with ubiquitin binding proteins. |
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| Reconstitution: | Note: It is recommended that the product be reconstituted at a moderately high protein concentration (>1mg/mL) and in the presence of buffer. If a more dilute reagent is required, the inclusion of an inert carrier protein (e.g. ovalbumin at 0.5-1.0mg/mL) in the storage buffer is recommended in order to minimise loss of the reagent due to absorption. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Store unopened vial at -20°C until required for use. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | Polyubiquitin chains, linked through specific lysine residues, are useful tools for investigating the specificity and reactivity of deubiquitinylating enzymes (DUBs) and the recognition and interaction of polyubiquitin modified proteins with ubiquitin binding domain (UBDs) containing proteins. |
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| Technical Info/Product Notes: | Note: Due to the methods of synthesis and purification used it is likely that batch to batch variations in the exact amounts of each chain size present may occur. Although variation is most unlikely to be of any significant value, those wishing to guarantee reproducibility of results over an extended period of time are advised to purchase sufficient material for ongoing studies. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot of K
48-linked polyubiquitin chains (Ub
2-16), detected by ubiquitin polyclonal antibody (Prod. No.
BML-UG9510 (1:1000))."
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Product Literature References
TDP-43 is a ubiquitylation substrate of the SCFcyclin F complex: S.L. Rayner, et al.; Neurobiol. Dis.
167, 105673 (2022),
Abstract;
The RNA helicase DHX16 recognizes specific viral RNA to trigger RIG-I-dependent innate antiviral immunity: A. Hage, et al.; Cell Rep.
38, 110434 (2022),
Abstract;
CYLD and the NEMO zinc finger regulate tumor necrosis factor signaling and early embryogenesis: Y. Zhao, et al.; J. Biol. Chem.
290, 22076 (2015),
Application(s): Western blot ,
Abstract;
Full Text
Ubiquitin and ubiquitin-modified proteins activate the Pseudomonas aeruginosa T3SS cytotoxin, ExoU: D.M. Anderson, et al.; Mol. Microbiol.
82, 1454 (2011),
Abstract;
Full Text
General Literature References
A HECT domain E3 enzyme assembles novel polyubiquitin chains: J. You & C.M. Pickart; J. Biol. Chem.
276, 19871 (2001),
Abstract;
E2/E3-mediated assembly of lysine 29-linked polyubiquitin chains: L.D. Mastrandrea, et al.; J. Biol. Chem.
274, 27299 (1999),
Abstract;
Inhibition of the 26 S proteasome by polyubiquitin chains synthesized to have defined lengths: J. Piotrowski, et al.; J. Biol. Chem.
272, 23712 (1997),
Abstract;
Metabolism of the polyubiquitin degradation signal: structure, mechanism, and role of isopeptidase T: K.D. Wilkinson, et al.; Biochemistry
34, 14535 (1995),
Abstract;
