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SUMO-1 (human), (recombinant) (biotin conjugate)

BML-UW0545-0100 100 µg 230.00 USD
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A useful reagent for studying SUMOylation in vitro. Represents a readily detectable alternative to radio-iodinated SUMO-1. Biotinylated SUMO-1 is activated by SUMO E1, a competent substrate for the SUMO E2 conjugating enzyme Ubc9. Can be conjugated to target proteins, in either a SUMO E3 ligase dependent or independent manner, as appropriate. The biotinyl moiety also provides a potential route to affinity purification of conjugated substrates (or thiol ester-linked enzymes) on avidin/streptavidin matrices.

Product Specification

Alternative Name:Sentrin-1, Small ubiquitin-related modifier 1
MW:~13.5kDa (based on Bt3-SUMO-1 as predominant form present).
Source:Produced in E. coli.
UniProt ID:P63165
Formulation:Lyophilized solid. Contains PBS.
Application Notes:Suggested uses:
  1. Ready detection of SUMO-1 modification of specific proteins in vitro.
  2. Demonstration that novel proteins are potential targets for SUMOylation under in vitro conditions.
  3. Purification of SUMO-1 conjugates via streptavidin affinity chromatography.
Reconstitution:Reconstitute with sterile distilled water.
Shipping:Shipped on Blue Ice
Short Term Storage:-20°C
Long Term Storage:-20°C
Use/Stability:Stable for at least 12 months after receipt when stored at -20°C.
Handling:Avoid freeze/thaw cycles.
Scientific Background:SUMO (small ubiquitin-related modifier) is a member of a family of ubiquitin-like proteins involved in the regulation of the cellular functions of a wide variety of proteins. Four members of the SUMO family have been described in vertebrates, SUMO-1 and the close homologues SUMO-2 and SUMO-3, with some 50% homology between SUMO-1 and SUMO-2/3.
Technical Info/Product Notes:Note: SUMO-1 contains 11 lysine residues, any/many of which are likely to be biotinylated in this product. These modification sites have not been mapped.
Regulatory Status:RUO - Research Use Only
SUMO-1 (human), (recombinant) (biotin conjugate) Western blot
Western blot of biotinylated-SUMO-1 (BML-UW0545) SUMOylation assay. Bt-SUMO-1 conjugated to RanGAP1 (BML-UW9755) in the presence of SUMO E1 (BML-UW9330) and Ubc9 (BML-UW9320). Bt-SUMO-1 conjugated species detected with streptavidin-HRP."
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SUMO-1 (human), (recombinant) (biotin conjugate) Western blot

General Literature References

Sumoylation of heterogeneous nuclear ribonucleoproteins, zinc finger proteins, and nuclear pore complex proteins: a proteomic analysis: T. Li, et al.; PNAS 101, 8551 (2004), Abstract;
In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2: T. Okuma, et al.; BBRC 254, 693 (1999), Abstract;

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