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SUMO-1 (93-97)-AMCA

BML-UW0500-1000 1 mg 197.00 USD
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SUMO1-AMCA is a synthetic AMCA-peptide substrate based on the C-terminal residues of SUMO1 and is a useful fluorogenic substrate for deSUMOylating enzymes, including SENP1 (BML-UW9760). It has particular utility for the study of deSUMOylating activity where continuous monitoring of activity is essential. It provides an alternative substrate to SUMO1-AMC.

Product Details

Formulation:Lyophilized solid.
Purity:≥95% (HPLC)
Application Notes:Useful fluorogenic substrate for deSUMOylating enzymes.

Typical assay conditions:
Assay substrate concentration: 12.5µM-100µM.
Enzyme concentrations: 0.5µM-4µM.
Release of AMCA fluorescence by deSUMOylating enzymes can be monitored using fluorimetry (380nm excitation and 460nm emission wavelengths).

  1. Substrate for deSUMOylating enzyme activity assays.
  2. Identification/confirmation of enzyme deSUMOylation activity.
  3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC/AMCA substrates (e.g. SUMO1-AMC, NEDD8-AMCA).
Solubility:Soluble in DMSO. Can be subsequently diluted in aqueous buffers.
Shipping:Blue Ice
Long Term Storage:-20°C
Use/Stability:Store solid at -20°C for up to twelve months. Solutions can be stored for up to three months at -20°C.
Handling:Avoid freeze/thaw cycles.
Regulatory Status:RUO - Research Use Only
SUMO-1 (93-97)-AMCA assay
DeSUMOylation Assay: 2µM SENP1 (Prod. No. BML-UW9760); 100µM SUMO1-AMCA (Prod. No. BML-UW0500); 50mM TRIS, pH 8.0, 20mM NaCl, 5mM DTT."
SUMO-1 (93-97)-AMCA hplc
HPLC trace
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SUMO-1 (93-97)-AMCA assay SUMO-1 (93-97)-AMCA hplc

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