SUMO-1 (93-97)-AMCA

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BML-UW0500-1000	1 mg	197.00 USD
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SUMO1-AMCA is a synthetic AMCA-peptide substrate based on the C-terminal residues of SUMO1 and is a useful fluorogenic substrate for deSUMOylating enzymes, including SENP1 (BML-UW9760). It has particular utility for the study of deSUMOylating activity where continuous monitoring of activity is essential. It provides an alternative substrate to SUMO1-AMC.

Sequence:	E⁹³QTGG⁹⁷-AMCA

MW:	747

Formulation:	Lyophilized solid.

Purity:	≥95% (HPLC)

Application Notes:	Useful fluorogenic substrate for deSUMOylating enzymes. Typical assay conditions: Assay substrate concentration: 12.5µM-100µM. Enzyme concentrations: 0.5µM-4µM. Release of AMCA fluorescence by deSUMOylating enzymes can be monitored using fluorimetry (380nm excitation and 460nm emission wavelengths). Uses: Substrate for deSUMOylating enzyme activity assays. Identification/confirmation of enzyme deSUMOylation activity. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC/AMCA substrates (e.g. SUMO1-AMC, NEDD8-AMCA).

Solubility:	Soluble in DMSO. Can be subsequently diluted in aqueous buffers.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Use/Stability:	Store solid at -20°C for up to twelve months. Solutions can be stored for up to three months at -20°C.

Handling:	Avoid freeze/thaw cycles.

Regulatory Status:	RUO - Research Use Only

DeSUMOylation Assay: 2µM SENP1 (Prod. No. BML-UW9760); 100µM SUMO1-AMCA (Prod. No. BML-UW0500); 50mM TRIS, pH 8.0, 20mM NaCl, 5mM DTT."

HPLC trace

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SENP1 (catalytic domain) (human), (recombinant) (GST-tag)

Produced in E. coli. | Print as PDF

BML-UW9760-0100

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