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[K¹¹R]SUMO-3 (human), (recombinant) (GST-tag)



BML-UW0385-0100	100 µg	89.00 USD
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The [K¹¹R] mutant of SUMO-3 is incapable of forming polySUMO-3 chains at Lys¹¹ and can be used to selectively mono-/multi-SUMOylate target proteins.

Product Specification

Alternative Name:	SMT3A, SMT3H1

MW:	~40kDa

Source:	Produced in E. coli BL21 (λDE3).

UniProt ID:	P55854

Formulation:	Liquid. In 50mM TRIS, pH 7.5, containing 150mM NaCl and 1mM DTT.

Purity:	≥95% (SDS-PAGE)

Purity Detail:	Purified by glutathione affinity chromatography.

Shipping:	Shipped on Dry Ice

Short Term Storage:	-80°C

Long Term Storage:	-80°C

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C.

Handling:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

Scientific Background:	SUMO (small ubiquitin-related modifier) is a member of a family of ubiquitin-like proteins involved in the regulation of the cellular functions of a wide variety of proteins. Four members of the SUMO family have been described in vertebrates, SUMO-1 and the close homologues SUMO-2 and SUMO-3, with some 50% homology between SUMO-1 and SUMO-2/3.

Regulatory Status:	RUO - Research Use Only

[K11R]SUMO-3 (human), (recombinant) (GST-tag) SDS-PAGE

Figure: SDS-PAGE image of [K¹¹R]SUMO-3, GST-tagged (Coomassie blue stained). Lane 1: MW markers (top to bottom) 200, 116, 97, 66, 55, 45, 36, 29, 24, 20, 14.2KDa. Lane 2: Prod. No. BML-UW0385 (4µg).

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General Literature References

A stress-dependent SUMO4 sumoylation of its substrate proteins: W. Wei, et al.; BBRC 375, 454 (2008), Abstract;

The SUMO isopeptidase Ulp2 prevents accumulation of SUMO chains in yeast: G.R. Bylebyl, et al.; J. Biol. Chem. 278, 44113 (2003), Abstract;

Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9: M.H. Tatham, et al.; J. Biol. Chem. 276, 35368 (2001), Abstract;

SUMO, ubiquitin’s mysterious cousin: S. Muller, et al.; Nat. Rev. Mol. Cell Biol. 2, 202 (2001), Abstract;

SUMO-1 conjugation in vivo requires both a consensus modification motif and nuclear targeting: M.S. Rodriguez, et al.; J. Biol. Chem. 276, 12654 (2001), Abstract;