The [K11R] mutant of SUMO-3 is incapable of forming polySUMO-3 chains at Lys11 and can be used to selectively mono-/multi-SUMOylate target proteins.
Product Specification
Alternative Name: | SMT3A, SMT3H1 |
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MW: | ~40kDa |
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Source: | Produced in E. coli BL21 (λDE3). |
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UniProt ID: | P55854 |
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Formulation: | Liquid. In 50mM TRIS, pH 7.5, containing 150mM NaCl and 1mM DTT. |
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Purity: | ≥95% (SDS-PAGE) |
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Purity Detail: | Purified by glutathione affinity chromatography. |
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Shipping: | Shipped on Dry Ice |
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Short Term Storage: | -80°C |
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Long Term Storage: | -80°C |
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Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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Scientific Background: | SUMO (small ubiquitin-related modifier) is a member of a family of ubiquitin-like proteins involved in the regulation of the cellular functions of a wide variety of proteins. Four members of the SUMO family have been described in vertebrates, SUMO-1 and the close homologues SUMO-2 and SUMO-3, with some 50% homology between SUMO-1 and SUMO-2/3. |
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Regulatory Status: | RUO - Research Use Only |
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Figure: SDS-PAGE image of [K11R]SUMO-3, GST-tagged (Coomassie blue stained). Lane 1: MW markers (top to bottom) 200, 116, 97, 66, 55, 45, 36, 29, 24, 20, 14.2KDa. Lane 2: Prod. No. BML-UW0385 (4µg).
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General Literature References
A stress-dependent SUMO4 sumoylation of its substrate proteins: W. Wei, et al.; BBRC
375, 454 (2008),
Abstract;
The SUMO isopeptidase Ulp2 prevents accumulation of SUMO chains in yeast: G.R. Bylebyl, et al.; J. Biol. Chem.
278, 44113 (2003),
Abstract;
Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9: M.H. Tatham, et al.; J. Biol. Chem.
276, 35368 (2001),
Abstract;
SUMO, ubiquitin’s mysterious cousin: S. Muller, et al.; Nat. Rev. Mol. Cell Biol.
2, 202 (2001),
Abstract;
SUMO-1 conjugation in vivo requires both a consensus modification motif and nuclear targeting: M.S. Rodriguez, et al.; J. Biol. Chem.
276, 12654 (2001),
Abstract;