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K29-only Ubiquitin (human), (recombinant) (untagged)

BML-UW0225-0001 1 mg 96.00 USD
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Product Specification

MW:~8.6 kDa
Source:Produced in E. coli.
UniProt ID:P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
Purity:≥95% (SDS-PAGE) (SDS-PAGE)
Purity Detail:Purified by perchloric acid treatment of a soluble lysate, followed by gradient elution from a cation exchange column.
Appearance:White solid.
Application Notes:For production of poly-ubiquitin chains via Lys29 only.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Lys29-only ubiquitin contains only a single lysine at position 29, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains only via Lys29. The ability to form an active thioester at the C-terminus is preserved, allowing, Lys29-only ubiquitin to be transferred to the lysines of substrate proteins.
Regulatory Status:RUO - Research Use Only

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