Fluorogenic substrate for deSUMOylating enzymes.SUMO-2-AMC is a sensitive fluorogenic substrate for deSUMOylating enzymes, including SENP1 (Prod. No. BML-UW9760) and SENP2 (Prod. No. BML-UW9765). It is a particularly useful reagent for the study of deSUMOylating activity where detection sensitivity or continuous monitoring of activity is essential.
Product Details
| Alternative Name: | Sentrin-2-AMC |
| |
| MW: | ~10.8kDa |
| |
| Source: | Produced in E. coli. |
| |
| UniProt ID: | P61956 |
| |
| Formulation: | Liquid. In DMSO. |
| |
| Purity: | ≥95% (HPLC) |
| |
| Application Notes: | Uses:
1. Substrate for deSUMOylating enzyme activity assays.
2. Identification/confirmation of enzyme deSUMOylation activity.
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC substrates (e.g. Ubiquitin-AMC, Prod. No. BML-SE211). |
| |
| Shipping: | Dry Ice |
| |
| Short Term Storage: | -80°C |
| |
| Long Term Storage: | -80°C |
| |
| Use/Stability: | Stable for at least 12 months after receipt when stored at -80°C. |
| |
| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
| |
| Technical Info/Product Notes: | Typical assay conditions: Assay substrate concentration: 10nM-1.0µM. Enzyme concentrations: 10nM-1µM. Release of AMC fluorescence by deSUMOylating enzymes can be monitored using fluorimetry (380nm excitation and 460nm emission wavelengths). |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
DeSUMOylation Assay: 1µM SENP1 CD, His
6-tagged (Prod. No.
BML-UW9760); 500nM SUMO-2-AMC (Prod. No. BML-UW0045); 50mM HEPES, pH7.8, 0.5mM EDTA, 1mM DTT."
Please mouse over
Related Products
