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BML-UW0040-0050 50 µg 563.00 USD
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Fluorogenic substrate for deSUMOylating enzymes. SUMO1-AMC is a sensitive fluorogenic substrate for deSUMOylating enzymes, including SENP1 (Prod. No. BML-UW9760) and SENP2 (Prod. No. BML-UW9765). It is a particularly useful reagent for the study of deSUMOylating activity where detection sensitivity or continuous monitoring of activity is essential.

Product Details

Alternative Name:Sentrin-1-AMC
Source:Produced in E. coli.
UniProt ID:P63165
Formulation:Liquid. In 50mM HEPES, pH 7.5, containing 100 mM NaCl.
Purity:≥95% (HPLC)
Application Notes:Uses:
1. Substrate for deSUMOylating enzyme activity assays.
2. Identification/confirmation of enzyme deSUMOylation activity.
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL-AMC substrates (e.g. Ubiquitin-AMC, Prod. No. BML-SE211).
Shipping:Dry Ice
Short Term Storage:-80°C
Long Term Storage:-80°C
Use/Stability:Stable for at least 12 months after receipt when stored at -80°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Technical Info/Product Notes:Typical assay conditions: Assay substrate concentration: 10nM-1.0µM. Enzyme concentrations: 10nM-1µM. Release of AMC fluorescence by deSUMOylating enzymes can be monitored using fluorimetry (380nm excitation and 460nm emission wavelengths).
Regulatory Status:RUO - Research Use Only
SUMO-1-AMC image
DeSUMOylation Assay: 1µM SENP1 CD, His6-tagged (Prod. No. BML-UW9760); 500nM SUMO-1-AMC (Prod. No. BML-UW0040); 50mM HEPES, pH7.8, 0.5mM EDTA, 1mM DTT."
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SUMO-1-AMC image

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