Immunoblotting standard. Whole cell extract of human Jurkat macrophage cells, induced to undergo apoptosis by the chemotherapeutic agent etoposide. Supplied in SDS-PAGE buffer, ready to use. Applications: WB control for PARP antibodies to detect intact 116 kDA PARP and 85 kDa apoptosis-related cleavage product. Also, for detection of -procaspase-3 processing to active enzyme using caspase-3 antibody; and pro-caspase-9. Recommended usage is 10 µl per lane.
Product Specification
| Formulation: | Liquid. Cell extract in SDS-PAGE sample buffer. The product is ready to use for SDS-PAGE. |
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| Applications: | WB
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| Application Notes: | Useful as a Western blotting control for poly(ADP-ribose) polymerase (PARP) antibodies (Prod. No. BML-SA250, BML-SA249 and BML-SA253), caspase-3 antibody (Prod. No. BML-SA320) and caspase-9 antibody(Prod. No. BML-SA321), or for use with other antibodies/proteins. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Regulatory Status: | RUO - Research Use Only |
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