Immunoblotting standard. Whole cell extract of human Jurkat macrophage cells, induced to undergo apoptosis by the chemotherapeutic agent etoposide. Supplied in SDS-PAGE buffer, ready to use. Applications: WB control for PARP antibodies to detect intact 116 kDA PARP and 85 kDa apoptosis-related cleavage product. Also, for detection of -procaspase-3 processing to active enzyme using caspase-3 antibody; and pro-caspase-9. Recommended usage is 10 µl per lane.
Product Specification
Formulation:
Liquid. Cell extract in SDS-PAGE sample buffer. The product is ready to use for SDS-PAGE.
Applications:
WB
Application Notes:
Useful as a Western blotting control for poly(ADP-ribose) polymerase (PARP) antibodies (Prod. No. BML-SA250, BML-SA249 and BML-SA253), caspase-3 antibody (Prod. No. BML-SA320) and caspase-9 antibody(Prod. No. BML-SA321), or for use with other antibodies/proteins.
