PLA2 fluorogenic substrate for use in assays with phospholipid and Ca2+. For use with purified enzyme, as this substrate is nonspecific. Ideal for drug discovery, HTS, and similar applications. Ex.: 360 nm, Em.: 460 nm
Product Details
| Alternative Name: | 7-Hydroxycoumarinyl-γ-linolenate |
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| Formula: | C27H34O4 |
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| MW: | 422.6 |
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| CAS: | 161180-12-7 |
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| Purity: | ≥98% (TLC) |
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| Appearance: | Pale yellow oil. |
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| Solubility: | Soluble in 100% ethanol (35mg/ml) or DMSO (50mg/ml). |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Store, as supplied, at -80°C for up to 6 months. Not stable in solution. To aliquot material: dissolve in methylene chloride, aliquot into amounts suitable for a single use, and evaporate off the solvent under a stream of nitrogen or argon gas. Once the solvent has been removed, aliquots should be tightly sealed, and immediately returned to -80°C, these aliquots may be stored for up to 3 months. Thaw and dissolve aliquots immediately before use. Do not allow material to stand at room temperature. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A continuous fluorescence-based assay for the human high-molecular-weight cytosolic phospholipase A2: Z. Huang, et al.; Anal. Biochem.
222, 110 (1994),
Abstract;
