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Dihydrosphingosine-1-phosphate, D-erythro

S1P receptor ligand
BML-SL143-0001 1 mg 201.00 USD
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Replaces Prod. #: ALX-306-016

Saturated analog of sphingosine-1-phosphate. Induces chemotaxis in HUVECs. May be used as a negative control for intracellular effects of S1P. Rapidly dephosphorylated by a sphingosine phosphatase in cultured human fibroblasts. Ligand for S1P (EDG) receptors.

Product Details

Appearance:White solid.
Solubility:Soluble in methanol/THF/water (60/30/10) (0.5mg/ml). Slightly soluble in DMSO, methanol or acetic acid.
Shipping:Ambient Temperature
Long Term Storage:-20°C
Use/Stability:Stable for at least 1 year after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 3 months at -20°C.
Protocol:For delivery of dihydrosphingosine-1-phosphate to cells, prepare a 125µM stock in 4mg/ml BSA as follows: Dissolve dihydrosphingosine-1-phosphate (HSPP) in methanol/THF/water (60/30/10) (0.5mg/ml). This may require boiling (75°C), with occasional replacement of evaporated methanol. Crushing of solid prior to addition of solvent and crushing and stirring during heating is helpful. Aliquot desired amounts of stock solution to tubes. Evaporate the solvent with a stream of nitrogen, swirling to deposit a thin film of HSPP on the inside of the tube. Aliquots may be stored at –20 °C at this point or dissolved with enough 4mg/ml BSA (fatty acid free Bovine Serum Albumin in water; 37°C, 30 min. with repeated vortexing) to make 125µM stock.
Regulatory Status:RUO - Research Use Only
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Product Literature References

Dual actions of sphingosine-1-phosphate: extracellular through the Gi-coupled receptor Edg-1 and intracellular to regulate proliferation and survival: J.R. Van Brocklyn, et al.; J. Cell. Biol. 142, 229 (1998), Abstract;
Structural requirements of sphingosylphosphocholine and sphingosine-1-phosphate for stimulation of activator protein-1 activity: A. Berger, et al.; Mol. Pharmacol. 50, 451 (1996), Abstract;
Sphinganine 1-phosphate metabolism in cultured skin fibroblasts: evidence for the existence of a sphingosine phosphatase: P.P Van Veldhoven & G.P. Mannaerts; Biochem. J. 299, 597 (1994), Abstract;

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