The purified protein (34 kDa) is highly activatable by treatment with particular active caspases (caspase-1 >> caspase-10 > caspase-8), while having low inherent activity, and little or no contamination with processed caspase-7. The selectivity of the activation process may provide the basis for assays which 1) distinguish death-receptor initiated apoptosis from that initiated via the mitochondrial/apoptosome pathway or 2) detect active caspase-1.
Product Specification
| MW: | 34 kDa |
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| Source: | Produced in E. coli. Truncated human procaspase-7 (aa 24-303). |
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| UniProt ID: | P55210 |
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| GenBank ID: | U40281 |
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| Formulation: | Liquid. In 50mM HEPES, pH 7.4, 100mM NaCl, 0.5% CHAPS, 10mM DTT, 1mM EDTA, 10% glycerol . |
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| Purity Detail: | Partially purified by single-step affinity chromatography and gel filtration. |
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| Activity: | Recombinant procaspase-7, expressed in E. coli without the N-terminal prodomain, is relatively slow to autoactivate. It can be activated in vitro by treatment with active recombinant caspases. |
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| Shipping: | Shipped on Dry Ice |
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| Long Term Storage: | -80°C |
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| Regulatory Status: | RUO - Research Use Only |
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