| Alternative Name: | Proprotein convertase subtilisin/kexin 9, Narc-1, Neural apoptosis related convertase 1 |
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| MW: | 72.6 kDa (predicted). Migrates as a doublet of 20kDa (pro domain) and 62kDa (mature form) in SDS-PAGE under reducing conditions. |
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| Source: | Produced in human cells. DNA sequence encoding the human proprotein convertase 9 precusor (Met1-Gln692) was expressed with a C-terminal polyhistidine tag. |
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| EC: | EC 3.4.21.- |
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| UniProt ID: | Q8NBP7 |
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| Gene/Protein Identifier: | NP_777596.2 (RefSeq) |
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| Formulation: | Lyophilized from a 0.2µm filtered solution of PBS, pH 7.4, containing 8% trehalose, 8% mannitol. |
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| Purity: | ≥97% (SDS-PAGE) |
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| Endotoxin Content: | <1.0 EU per µg protein (LAL test) |
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| Activity: | Measure by its ability to bind with human LDLR in a functional ELISA: Immobilized recombinant human PCSK9 at 10µg/ml (100µl/well) can
bind biotinylated human LDLR. The EC50 of biotinylated human LDLR is 0.61µg/ml. |
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| Application Notes: | Can be used for binding studies with LDL/VLDL/ApoE receptor and Annexin A2, to cleave target substrates and to screen for inhibitors that block PCSK9 binding to lipoprotein receptors. Can be used in tissue cultures. |
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| Shipping: | Shipped on Dry Ice |
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| Short Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Scientific Background: | PCSK9 is a circulating glycosylated serine protease expressed primarily in kidney, liver, cerebellum, and small intestine. In order for secretion to occur, PCSK9 cleaves its own prodomain, which remains associated and largely inhibits further proteolytic activity. The major function of PCSK9 appears to be regulation of circulating cholesterol levels through its binding of the LDL receptor, resulting in degradation of the LDLR. siRNA inhibition of PCSK9 expression lowers plasma PCSK9, apoB, and LDL cholesterol. PCSK9 may also be involved in Alzheimer’s disease due to its requirement for degradation of BACE1. NOTE: There are many polymorphisms in the PCSK9 gene, some of which cause hyper- or hypocholesterolemia. |
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| Regulatory Status: | RUO - Research Use Only |
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