Deacetylates lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.
Product Details
| Alternative Name: | Histone deacetylase 11 |
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| MW: | ~39 kDa |
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| Source: | Produced in Sf9 insect cells. HDAC11 from human cDNA, transcript variant 1 (identical to GenBank accession NM_024827) is fused at the N-terminus to a His-tag. Produced in a baculovirus expression system. |
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| UniProt ID: | Q96DB2 |
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| Formulation: | Liquid. In 50mM TRIS, pH 8.0, containing 138mM NaCl and 10% glycerol. |
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| Purity Detail: | Partially purified by single-step affinity chromatography and gel filtration. |
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| Biological Activity: | Hydrolysis of N-ε-acetyl-lysine residues of histones. |
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| Specific Activity: | ≥2U/µg. One unit is defined as the amount of enzyme that deacetylates 1 pmol/min of FLUOR DE LYS®-SIRT1 substrate (Prod. No. BML-KI177) at 37°C / 500µM. |
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| Application Notes: | Useful for studies of enzyme kinetics, modulator activity, drug discovery. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable on ice for approx. 1h. Snap freezing in liquid nitrogen is recommended if refreezing of remaining, undiluted enzyme is necessary. Freezing and storage of diluted enzyme is not recommended. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | While the other ten human HDACs fall into either the class I (HDACs 1-3, 8) or class II (HDACs 4-7, 9, 10) homology groups, HDAC11 is the sole human representative in class IV.In contrast to the ubiquitous expression of most human HDACs, high level expression of HDAC11 is limited, in normal human tissues, to brain, heart, skeletal muscle, kidney and testis, suggesting possible tissue-specific or developmental functions. HDAC11 appears to play a role in neural cell maturation, particularly that of oligodendrocytes. HDAC11 deacetylation of histone H3 lysines 9 and 14 in the myelin basic protein and proteolipid protein genes stimulates their expression in oligodendrocytes, a notable example in which histone deacetylation, frequently associated with gene silencing, may in some cases have the opposite effect. HDAC11 negatively regulates IL-10 expression in antigen presenting cells (APCs), thus influencing whether APCs induce T cell activation or tolerance. Elevated HDAC11 expression is seen in a number of cancer cell lines. In myeloid leukemia lines, several HDAC inhibitors strongly induce HDAC11 expression, although the induction is more rapid with some inhibitors (TSA, SAHA) than others (butyrate, valproate). Such effects may be important to consider in the development and clinical application of HDAC inhibitors as cancer therapeutics. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A novel class of small molecule inhibitors of HDAC6: E.S. Inks, et al.; ACS Chem. Biol.
7, 331 (2011),
Abstract;
General Literature References
Histone deacetylase 11 regulates oligodendrocyte-specific gene expression and cell development in OL-1 oligodendroglia cells.: H. Liu, et al.; Glia
57, 1 (2009),
Abstract;
The histone deacetylase HDAC11 regulates the expression of interleukin 10 and immune tolerance.: A. Villagra, et al.; Nat. Immunol.
10, 92 (2009),
Abstract;
Developmental expression of histone deacetylase 11 in the murine brain.: H. Liu, et al.; J. Neurosci. Res.
86, 537 (2008),
Abstract;
Full Text
Histone deacetylases in acute myeloid leukaemia show a distinctive pattern of expression that changes selectively in response to deacetylase inhibitors.: C. A. Bradbury, et al.; Leukemia
19, 1751 (2005),
Abstract;
Molecular evolution of the histone deacetylase family: functional implications of phylogenetic analysis.: I. V. Gregoretti, et al.; J. Mol. Biol.
338, 17 (2004),
Abstract;
Cloning and functional characterization of HDAC11, a novel member of the human histone deacetylase family.: L. Gao, et al.; J. Biol. Chem.
277, 25748 (2002),
Abstract;
Full Text
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