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Insulin receptor β (human), (recombinant) (His-tag)

 
BML-SE516-0020 20 µg 374.00 USD
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The His-tagged protein, in contrast to the dimeric GST-tagged recombinant enzyme (SE-195), is monomeric. Under ordinary conditions, the monomeric enzyme autophosphorylates to only half the extent of the dimeric construct and consequently has 10-100 fold lower activity with exogenous peptide substrates. It can, however, be fully activated by autophosphorylation in the presence of an aggregating agent such as poly-L-lysine.

Product Specification

Alternative Name:IRKβ
 
MW:49 kDa
 
Source:Produced in insect cells. The cytoplasmic (kinase) domain of human insulin receptor is fused to a His-tag. Produced in a baculovirus expression system.
 
UniProt ID:P06213
 
Formulation:Liquid. In 50mM TRIS, pH 7.5, and 1mM DTT.
 
Purity Detail:Partially purified by single-step affinity chromatography and gel filtration.
 
Specific Activity:≥100 pmol/min/µg at 30°C using ‘IR0’ peptide substrate, (Prod. No. BML-P314) (200 µM).
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Regulatory Status:RUO - Research Use Only
 

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