Product Details
| Alternative Name: | E1A binding protein p300, Histone acetyltransferase p300 |
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| MW: | 45.1 kDa |
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| Source: | Produced in E. coli. Catalytic domain (aa 1284-1673) of human p300. |
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| UniProt ID: | Q09472 |
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| Formulation: | Liquid. In 50mM TRIS/HCl, pH 8.0, containing 0.1mM EDTA, 10% glycerol. |
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| Purity Detail: | Partially purified by single-step affinity chromatography and gel filtration. |
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| Specific Activity: | ≥250 pmol/min/µg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386 (Prod. No. BML-P198). CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis(2-nitrobenzoic acid)). |
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| Application Notes: | Can be used to study p300 kinetics and regulation, for screening for inhibitors or activators and for radiolabeling of proteins or peptides with, for example, [3H]-Acetyl CoA. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | Although originally termed histone acetyltransferases (HATs) for their lysine acetylation activity on histone N-terminal tails, p300 and its paralogue, CBP, have been shown to acetylate a variety of non-histone proteins including p53, DNA polymerase β and nuclear import factors. p300/CBP acetylations play regulatory roles in transcription, DNA repair and replication, the cell cycle, p53 turnover, and nuclear import. In addition to functions which overlap with those of CBP, p300 has a number of unique functions, including a role in the induction of p21Waf1/Cip1 cell cycle inhibitor. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Cyclophilin A Is Not Acetylated at Lysine-82 and Lysine-125 in Resting and Stimulated Platelets: A. Rosa, et al.; Int. J. Mol. Sci.
23, 1469 (2022),
Abstract;
SIRT1 modulates cell cycle progression by regulating CHK2 acetylation-phosphorylation: W. Zhang, et al.; Cell Death Differ.
27, 482 (2020),
Abstract;
Full Text
Interplay of Histone Marks with Serine ADP-Ribosylation: E. Bartlett, et al.; Cell Rep.
24, 3488 (2018),
Abstract;
Full Text
Inhibition of Wnt/β-Catenin pathway and Histone acetyltransferase activity by Rimonabant: a therapeutic target for colon cancer: M.C. Proto, et al.; Sci. Rep.
7, 11678 (2017),
Abstract;
Full Text
The histone acetyltransferase p300 inhibitor C646 reduces pro-inflammatory gene expression and inhibits histone deacetylases: T. Bosch, et al. ; Biochem. Pharmacol.
102, 130 (2016),
Application(s): Incubation,
Abstract;
A Novel Cell-Permeable, Selective, and Noncompetitive Inhibitor of KAT3 Histone Acetyltransferases from a Combined Molecular Pruning/Classical Isosterism Approach: C. Milite, et al.; J. Med. Chem.
58, 2779 (2015),
Application(s): Surface Plasmon Resonance, Assay,
Abstract;
Full Text
Acetylation of Beclin 1 inhibits autophagosome maturation and promotes tumour growth: T. Sun, et al.; Nat. Commun.
6, 7215 (2015),
Application(s): Incubation,
Abstract;
Full Text
Histone deacetylase 6 (HDAC6) promotes the pro-survival activity of 14-3-3ζ via deacetylation of lysines within the 14-3-3ζ binding pocket: J.B. Mortenson, et al.; J. Biol. Chem.
290, 12487 (2015),
Abstract;
Full Text
Identification and characterization of small-molecule inhibitors of lysine acetyltransferases: D. Liao; Methods Mol. Biol.
1238, 539 (2015),
Abstract;
MuLV IN mutants responsive to HDAC inhibitors enhance transcription from unintegrated retroviral DNA: W.M. Schneider, et al.; Virology
426, 188 (2012),
Abstract;
Full Text
General Literature References
Acetylation of p53 at lysine 373/382 by the histone deacetylase inhibitor depsipeptide induces expression of p21(Waf1/Cip1): Y. Zhao, et al.; Mol. Cell. Biol.
26, 2782 (2006),
Abstract;
CBP and p300: HATs for different occasions: E. Kalkhoven; Biochem. Pharmacol.
68, 1145 (2004),
Abstract;
CBP and p300: HATs for different occasions: E. Kalkhoven; Biochem. Pharmacol.
68, 1145 (2004),
Abstract;
Regulation of the p300 HAT domain via a novel activation loop: P.R. Thompson, et al.; Nat. Struct. Mol. Biol.
11, 308 (2004),
Abstract;
Acetylation regulates the DNA end-trimming activity of DNA polymerase beta: S. Hasan, et al.; Mol. Cell
10, 1213 (2002),
Abstract;
Histone acetyltransferases: S.Y. Roth, et al.; Annu. Rev. Biochem.
70, 81 (2001),
Abstract;
Acetylation of importin-alpha nuclear import factors by CBP/p300: A.J. Bannister, et al.; Curr. Biol.
10, 467 (2000),
Abstract;
Distinct roles of the co-activators p300 and CBP in retinoic-acid-induced F9-cell differentiation: H. Kawasaki, et al.; Nature
393, 284 (1998),
Abstract;
Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain: W. Gu & R.G. Roeder; Cell
90, 595 (1997),
Abstract;
