| Alternative Name: | pp60src |
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| MW: | ~83kDa |
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| Source: | Produced in E. coli. |
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| UniProt ID: | P12931 |
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| Formulation: | Liquid. In 50mM TRIS-HCl, pH 7.5, containing 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Application Notes: | Useful for kinetic and functional studies, phosphorylation of target substrates, drug screening. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | After initial defrost, aliquot product into individual tubes and refreeze the remaining, unused enzyme quickly by snap-freezing in a dry/ice ethanol bath or liquid nitrogen, if possible. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Scientific Background: | Src, a non-receptor type tyrosine kinase, is a proto-oncogene that plays key roles in cell morphology, motility, proliferation, and survival. Src is overexpressed and activated in a large number of human cancers. Oncogenic forms of Src alter cell structure and affect thin filaments and adhesion networks that control cell migration. Src also influences the RhoA-ROCK pathway that controls actin polymerization, the STAT family of transcription factors which are involved in tumorigenesis and the Cbl ubiquitin ligase that controls Src protein levels. |
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| Regulatory Status: | RUO - Research Use Only |
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