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PTP1B (human), (recombinant)

 
BML-SE332-0050 50 µg 436.00 USD
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Product Specification

Alternative Name:Protein tyrosine phosphatase 1B
 
MW:~37.4kDa
 
Source:Produced in E. coli. Human PTP1B (protein tyrosine phosphatase 1B) (aa 1-322).
 
UniProt ID:P18031
 
Formulation:Liquid. In 50mM HEPES, pH7.2, containing 1mM EDTA, 5mM DTT, and 0.05% NP-40.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Specific Activity:≥20 µmol/min/mg at 30°C using 0.15mM of phosphopeptide substrate based on EGFR residues 988-998 (Prod. No. BML-P323) in 50mM HEPES, pH 7.2, 1mM EDTA, 1mM DTT, 0.05% NP-40, and 0.2µg/µl BSA.
 
Application Notes:Useful for the study of tyrosine phosphatase kinetics, substrate specificity and for screening inhibitors.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:PTP1B is the ubiquitous, prototypical nontransmembrane PTP originally identified in human placenta.
 
Regulatory Status:RUO - Research Use Only
 
PTP1B (human), (recombinant) SDS-PAGE
SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 1 µg, Lane 3: 2 µg, Lane 4: 5 µg PTP1B. The higher molecular weight bands are BSA included for stability in storage buffer.
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PTP1B (human), (recombinant) SDS-PAGE

Product Literature References

Cell penetrable, clickable and tagless activity-based probe of human cathepsin L: D. Dana, et al.; Bioorg. Chem. 85, 505 (2019), Application(s): Activity assay, Abstract;
Absolute Structures of Wedelolide Derivatives and Structure-Activity Relationships of Protein Tyrosine Phosphatase 1B Inhibitory ent-Kaurene Diterpenes from Aerial Parts of Wedelia spp. Collected in Indonesia and Japan: D.B. Abdjul, et al.; Chem. Pharm. Bull. (Tokyo) 66, 682 (2018), Abstract; Full Text
Cladosporamide A, a new protein tyrosine phosphatase 1B inhibitor, produced by an Indonesian marine sponge-derived Cladosporium sp: H. Rotinsulu, et al.; J. Nat. Med. 72, 779 (2018), Abstract;
High-Resolution PTP1B Inhibition Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of PTP1B Inhibitors from Miconia albicans: R. de Cassia Lemos Lima, et al.; Molecules 23, E1755 (2018), Abstract; Full Text
Quadruple high-resolution α-glucosidase/α-amylase/PTP1B/radical scavenging profiling combined with high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy for identification of antidiabetic constituents in crude root bark of Morus alba L: Y. Zhao, et al.; J. Chromatogr. A 1556, 55 (2018), Abstract;
Protein tyrosine phosphatase 1B inhibitory properties of seco-cucurbitane triterpenes obtained from fruiting bodies of Russula lepida: W. Maarisit, et al.; J. Nat. Med. 71, 334 (2017), Abstract;
Prunin is a highly potent flavonoid from Prunus davidiana stems that inhibits protein tyrosine phosphatase 1B and stimulates glucose uptake in insulin-resistant HepG2 cells: H.A. Jung, et al.; Arch. Pharm. Res. 40, 37 (2017), Abstract;
PTP1B inhibitors from the seeds of Iris sanguinea and their insulin mimetic activities via AMPK and ACC phosphorylation: J.L. Yang, et al.; Bioorg. Med. Chem. Lett. 27, 5076 (2017), Abstract;
Coumarins from Angelica decursiva inhibit α-glucosidase activity and protein tyrosine phosphatase 1B: M.Y. Ali, et al.; Chem. Biol. Interact. 252, 93 (2016), Application(s): Used in treatment of Diabetes mellitus, Abstract;
High-resolution PTP1B inhibition profiling combined with high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy: Proof-of-concept and antidiabetic constituents in crude extract of Eremophila lucida: Y. Tahtah, et al.; Fitoterapia 110, 52 (2016), Application(s): PTP1B enzyme was used for the microplate-based PTP1B inhibition assay, Abstract;
Identification and evaluation of magnolol and chrysophanol as the principle protein tyrosine phosphatase-1B inhibitory compounds in a Kampo medicine, Masiningan: T. Onoda, et al.; J. Ethnopharmacol. 186, 84 (2016), Application(s): PTP1B inhibitory activity, Abstract;
Isopetrosynol, a New Protein Tyrosine Phosphatase 1B Inhibitor, from the Marine Sponge Halichondria cf. panicea Collected at Iriomote Island: D.B. Abdjul, et al.; Chem. Pharm. Bull. 64, 733 (2016), Application(s): Protein - used with inhibitory assay and assessing biological activity, Abstract; Full Text
Molecular Modeling of Licochalcone E as Protein Tyrosine Phosphatase 1B Inhibitor: E. Kim, et al.; Bull. Korean Chem. Soc. 37, 2102 (2016),
Protein tyrosine phosphatase 1B and α-glucosidase inhibitory activities of Pueraria lobata root and its constituents: S.H. Seong, et al.; J. Ethnopharmacol. 194, 706 (2016), Application(s): Inhibitory activity and enzyme kinetic analysis, coral, Abstract;
Structures and biological activities of triterpenes and sesquiterpenes obtained from Russula lepida: J.S. Lee, et al.; Phytochemistry 127, 63 (2016), Application(s): Fluorescence microscopy assay, Abstract;
The potent inhibitors of protein tyrosine phosphatase 1B from the fruits of Melaleuca leucadendron: A. Saifudin, et al.; Pharmacognosy Res. 8, S38 (2016), Application(s): Protein tyrosine phosphatase 1B inhibitory activity, Abstract; Full Text
Antihyperglycemic and antioxidant activities of twig extract from Cinnamomum osmophloeum: G.M. Lin, et al.; J. Tradit. Complement. Med. 6, 281 (2015), Application(s): Assay, Abstract; Full Text
Protein tyrosine phosphatase 1B inhibitory activity of alkaloids from Rhizoma Coptidis and their molecular docking studies: J.S. Choi, et al.; J. Ethnopharmacol. 171, 28 (2015), Application(s): Inhibition Assay, Abstract;
Selaginellin and biflavonoids as protein tyrosine phosphatase 1B inhibitors from Selaginella tamariscina and their glucose uptake stimulatory effects: P. H. Nguyen, et al.; Bioorg. Med. Chem. 23, 3730 (2015), Application(s): Assay, Abstract;
Trichoketides A and B, two new protein tyrosine phosphatase 1B inhibitors from the marine-derived fungus Trichoderma sp: H. Yamazaki, et al.; J. Antibiot. (Tokyo) 68, 628 (2015), Application(s): Inhibitory Assay, Abstract;
Two new diphenyl ethers from Acanthopanax senticosus (Rupr. & Maxim.) Harms with PTP1B inhibitory activity: N. Li, et al.; Phytochem. Lett. 13, 286 (2015),
Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae): S.H. Tiong, et al.; Fitoterapia 102, 182 (2015), Application(s): Assay, Abstract;
Bioactive compounds from culinary herbs inhibit a molecular target for type 2 diabetes management, dipeptidyl peptidase IV: A.M. Bower, et al.; J. Agric. Food Chem. 62, 6147 (2014), Abstract;
Conjugation-free, visual, and quantitative evaluation of inhibitors on protein tyrosine kinases and phosphatases with a luminescent Tb(III) complex: H. Akiba, et al.; Anal. Bioanal. Chem. 406, 2957 (2014), Abstract;
Development of a highly potent, selective, and cell-active Inhibitor of cysteine cathepsin L-A hybrid design approach: D. Dana, et al.; Chem. Commun. (Camb.) 50, 10875 (2014), Abstract;
Development of cell-active non-peptidyl inhibitors of cysteine cathepsins: D. Dana, et al.; Bioorg. Med. Chem. 21, 2975 (2013), Abstract;
Identification of a second aryl phosphate-binding site in protein-tyrosine phosphatase 1B: a paradigm for inhibitor design: Y.A. Puius, et al.; PNAS 94, 13420 (1997), Abstract;
Direct binding of the proline-rich region of protein tyrosine phosphatase 1B to the Src homology 3 domain of p130(Cas): F. Liu, et al.; J. Biol. Chem. 271, 31290 (1996), Abstract;

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