| Alternative Name: | Receptor-type protein tyrosine phosphatase β, HPTPβ |
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| MW: | 64 kDa |
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| Source: | Produced in E. coli. Catalytic domain (aa 1675-1996) of human PTPRB fused to an N-terminal GST-tag. |
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| UniProt ID: | P23467 |
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| Formulation: | Liquid. In 25 mM Tris-HCl, pH 8.0, containing 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM dithiothreitol, 10 mM glutathione. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Specific Activity: | 40 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50mM HEPES, pH 7.4, 2mM EDTA, 3mM DTT, 100mM NaCl, 50mM pNPP. |
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| Application Notes: | Useful for enzyme kinetics studies, dephosphorylation of target substrates and drug discovery. Can be used to remove phosphate specifically from tyrosine residues in proteins. Rate of dephosphorylation depends on PTPRB substrate preference. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | When stored under the conditions described, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing, could lead to loss of activity. |
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| Regulatory Status: | RUO - Research Use Only |
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