Product Details
| Alternative Name: | CPP32, Yama, Apopain |
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| MW: | 17 + 12 kDa |
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| Source: | Produced in E. coli. Caspase-3 (CPP32; Yama; apopain) from human cDNA. The enzyme was cleaved and activated from the proenzyme |
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| UniProt ID: | P42574 |
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| Formulation: | Liquid. In 50mM HEPES, pH 7.4, 100mM sodium chloride, 0.5% CHAPS, 1mM EDTA, 10% glycerol and 10mM dithiothreitol. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Activity: | 100 U/µl |
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| Specific Activity: | One U=1 pmol/min using Ac-DEVD-pNA (200 µM; Prod. No. ALX-260-033) as substrate at 30°C |
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| Application Notes: | Can be used to study enzyme regulation and kinetics, cleave target substrates and screen for inhibitors. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | The major effector caspase of apoptosis, caspase-3 is responsible for cleavage of multiple cellular targets. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Conjunctival epitheliopathy induced by topical exposure to bacterial peptidoglycan, muramyl dipeptide: M.P. Langford, et al.; Exp. Eye Res.
227, 109383 (2023),
Abstract;
Caspase-10 affects the pathogenesis of primary biliary cholangitis by regulating inflammatory cell death: M. Cho, et al.; J. Autoimmun.
133, 102940 (2022),
Abstract;
Enzyme-triggered compound release by using functionalized derivatives using antimicrobial peptide: S. Mizukami, et al.; Chem. Sci.
8, 3047 (2017),
Abstract;
Full Text
Approaches to design non-covalent inhibitors for human granzyme B (hGrB): M.S. Kim, et al.; Org. Biomol. Chem.
12, 8952 (2014),
Abstract;
Propeptide-mediated inhibition of cognate gingipain proteinases: N.L. Huq, et al.; PLoS One
8, e65447 (2013),
Abstract;
Vulnerable windows for developmental ethanol toxicity in the Japanese medaka fish (Oryzias latipes): S.L. Oxendine, et al.; Aquat. Toxicol.
80, 396 (2006),
Abstract;
Protein kinase C delta blocks immediate-early gene expression in senescent cells by inactivating serum response factor: K. Wheaton, et al.; Mol. Cell Biol.
24, 7298 (2004),
Abstract;
Full Text
Cross-talk between calpain and caspase proteolytic systems during neuronal apoptosis: R.W. Neumar, et al.; J. Biol. Chem.
278, 14162 (2003),
Abstract;
Full Text
A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N. A. Thornberry et al.; J. Biol. Chem.
272, 17907 (1997),
Abstract;
Structure of recombinant human CPP32 in complex with the tetrapeptide acetyl-Asp-Val-Ala-Asp fluoromethyl ketone: P. R. E. Mittl et al.; J. Biol. Chem.
272, 6539 (1997),
Abstract;
Substrate specificities of caspase family proteases: R. V. Talanian et al.; J. Biol. Chem.
272, 9677 (1997),
Abstract;
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