| BML-SA676-0200 | 200 µl | 494.00 USD |  |
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| Alternative Name: | Stromal interaction molecule 1 |
| Host: | Rabbit |
| Immunogen: | Synthetic peptide corresponding to aa 56-69 (C56HSEDEKLSFEAVR69) of STIM1. |
| UniProt ID: | Q13586 |
| Species reactivity: | Human, Rat |
| Applications: | Flow Cytometry, ICC, IHC, WB |
| Recommended Dilutions/Conditions: | Flow Cytometry (5-10µg/5x105 cells) Immunocytochemistry (1:20-1:100) Western blot (1:500-1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Blocking Peptide: | 40μg included. Lyophilized. Reconstitute with 100μl distilled water. |
| Purity Detail: | Affinity purified. |
| Formulation: | Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. |
| Reconstitution: | Reconstitute with 50μl or 200μl distilled water. Further dilutions should be made with medium containing a carrier protein such as 1% BSA. |
| Use/Stability: | The antibody solution can be stored at +4°C for up to 2 weeks. |
| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
| Shipping: | Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation. The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest to researchers. Several key players of the store operated complex have been identified; the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum Ca2+ sensor3,4. The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types. |
| Technical Info/Product Notes: | Preadsorption control: Use 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments. |
| Regulatory Status: | RUO - Research Use Only |
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