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Orai1 (extracellular) (human) polyclonal antibody

BML-SA674-0200 200 µl 649.00 USD
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Product Details

Alternative Name:Calcium release-activated calcium channel protein 1, Transmembrane protein 142A
Immunogen:Synthetic peptide corresponding to aa 203-214 (K203KQPGQPRPTSK214) of human Orai1.
UniProt ID:Q96D31
Species reactivity:Human
Crossreactivity:Does not cross-react with mouse or rat Orai1.
Applications:Flow Cytometry, WB
Recommended Dilutions/Conditions:Western Blot (1:200)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Affinity purified.
Formulation:Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide.
Reconstitution:Reconstitute with 50μl or 200μl distilled water. Further dilutions should be made with medium containing a carrier protein such as 1% BSA.
Use/Stability:The antibody solution can be stored at +4°C for up to 2 weeks.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation. The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest to researchers. Several key players of the store operated complex have been identified. Orai1 (also known as CRACM1) acts as the store operated calcium channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor. The formation of functional channels by Orai1 requires the presence of both Orai1 and STIM1 proteins working as a complex that involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum.
Technical Info/Product Notes:Preadsorption control: 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min.  Use the supernatants from tubes 1 and 2 for parallel experiments.
Regulatory Status:RUO - Research Use Only
Orai1 (extracellular) (human) polyclonal antibody Western blot
Figure: Western blot analysis of Jurkat cell lysate. Lane 1: PAb to Orai1 (Extracellular) (human) (Prod. No. BML-SA674) Lane 2: PAb to Orai1 (Extracellular) (human) preincubated with the control peptide. 
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Orai1 (extracellular) (human) polyclonal antibody Western blot

General Literature References

A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function: S. Feske, et al.; Nature 441, 179 (2006), Abstract;
Ca2+ store depletion causes STIM1 to accumulate in ER regions closely associated with the plasma membrane: M.M. Wu, et al.; J. Cell Biol. 174, 803 (2006), Abstract; Full Text
Calcium signaling in non-excitable cells: Ca2+ release and influx are independent events linked to two plasma membrane Ca2+ entry channels: R. Chakrabarti & R. Chakrabarti; J. Cell Biochem. 99, 1503 (2006), Abstract;
Calcium: You lose some, you gain some: J. Pickett; Nature Rev. Mol. Cell Biol. 7, 794 (2006),
The elementary unit of store-operated Ca2+ entry: local activation of CRAC channels by STIM1 at ER-plasma membrane junctions: R.M. Luik, et al.; J. Cell. Biol. 174, 815 (2006), Abstract; Full Text
Stability and instability of regulation of intracellular calcium: D.A. Eisner, et al.; Exp. Physiol. 90, 3 (2005), Abstract; Full Text

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