Proteinase-activated receptor-2 polyclonal antibody



BML-SA651-0200	200 µl	575.00 USD
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Product Details

Alternative Name:	PAR-2

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to aa 368-382 of rat PAR-2 (proteinase-activated receptor-2).

UniProt ID:	Q63645

Species reactivity:	Human, Mouse, Rat

Applications:	ICC, WB

Formulation:	Lyophilized. In PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Scientific Background:	PAR-2 belongs to a four member family of G protein-coupled receptors (PAR-1 to -4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this modality of activation, a specific proteinase cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This cleavage results in the creation of a new N-terminal sequence (tethered ligand), which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways^1-3 Different PARs are activated by different proteinases. Hence, PAR-1 is activated by thrombin as are PAR-3 and PAR-4, while PAR-2 is activated by trypsin^1-3. PAR-2 can be also cleaved and activated by other proteinases such as tryptase, membrane-type serine protease 1, and proteinase 3^1-3 PAR-2-mediated intracellular signaling has not been clearly elucidated, but activators of PAR-2 induce generation of IP3 and mobilization of intracellular Ca²⁺. Activation of the ERK1/2 and NF-kB intracellular pathways following PAR-2 activation has been also described^1-3 Tissue distribution of PAR-2 is wide with the highest expression levels found in pancreas, small intestine, liver and kidney. In addition, PAR-2 expression was observed in dorsal root ganglion neurons, smooth muscle cells and endothelial cells. The physiological role of PAR-2 is not yet clearly understood, however studies using PAR-2 knockout mice have suggested roles in the cardiovascular, pulmonary and gastrointestinal systems. Several studies suggest that PAR-2 plays a central role in inflammatory diseases and nociceptive pain transduction; hence PAR-2 blockers have been proposed to be useful in the therapeutic control of inflammation and pain^1-3.

Regulatory Status:	RUO - Research Use Only

Product Literature References

Presenilin1 promotes trypsin-induced neuroprotection via the PAR2/ERK signaling pathway. Effects of presenilin1 FAD mutations: A.M. Nikolakopoulou, et al.; Neurobiol. Aging 42, 41 (2016), Application(s): Treatment with inhibitors and cell lysates, Abstract;