| Alternative Name: | MC3R |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 102-117 of rat MC3R (melanocortin receptor 3). |
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| UniProt ID: | P32244 |
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| Species reactivity: | Human, Mouse, Rat
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| Applications: | Flow Cytometry, IHC, WB
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| Blocking Peptide: | Included: Melanocortin Receptor 3 Control Peptide (40µg). Supplied lyophilized.
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| Purity Detail: | Affinity purified IgG. |
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| Formulation: | Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide. |
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| Reconstitution: | To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. To lyophilized peptide, add 100µl of deionized water. |
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| Use/Stability: | Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The melanocortin receptors are members of the G protein-coupled receptor superfamily. Their ligands, the melanocortins, are peptides consisting of µ-, β- and γ-melanocyte stimulating hormone and adrenocorticotropic hormone. MC3R binds all melanocortins with equal affinity. Agouti-related protein (AGRP) is the high affinity endogenous antagonist of MC3R. It is expressed in the brain, placenta, and gut tissues, but not in the adrenal cortex or melanocytes. |
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| Technical Info/Product Notes: | PREADSORPTION CONTROL: 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium aide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments. |
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| Regulatory Status: | RUO - Research Use Only |
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