| BML-SA612-0050 | 50 µl | 373.00 USD |  |
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| BML-SA612-0200 | 200 µl | 571.00 USD | |
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| Alternative Name: | Glutamate receptor 1, AMPA-selective glutamate receptor 1, Glutamate receptor ionontropic AMPA 1 |
| Host: | Rabbit |
| Immunogen: | Synthetic peptide corresponding to aa 271-285 of rat GluR1 (GRIA1). |
| UniProt ID: | P19490 |
| Species reactivity: | Mouse, Rat |
| Applications: | IHC, WB |
| Recommended Dilutions/Conditions: | Immunohistochemistry (1:100) Western Blot (1:200) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Blocking Peptide: | Included: GluR1 (extracellular) Control Peptide (100µg). Supplied lyophilized. |
| Purity Detail: | Affinity purified |
| Formulation: | Lyophilized from PBS, pH 7.4, 1% BSA and 0.025% sodium azide. |
| Reconstitution: | To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. Further dilutions should be made using a carrier protein such as 1% BSA. To lyophilized control peptide, add 100µl of distilled water. |
| Use/Stability: | Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C. |
| Shipping: | Shipped on Blue Ice |
| Long Term Storage: | -20°C |
| Scientific Background: | GluR receptors are permeable to cations Na and K and Ca2+. Gating of Glu receptors by glutamate is extremely fast and therefore the Glu receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. |
| Technical Info/Product Notes: | PREADSORPTION CONTROL: 1µg control peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments. |
| Regulatory Status: | RUO - Research Use Only |
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