Product Specification
Alternative Name: | Metabotropic glutamate receptor 1, GRM1 |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to aa 501-516 of rat mGluR1 (GRM1). |
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UniProt ID: | P23385 |
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Species reactivity: | Human, Mouse, Rat
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Applications: | Flow Cytometry, ICC, IP, WB
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Formulation: | Lyophilized from PBS, pH 7.4, 1% BSA and 0.05% sodium azide. |
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Reconstitution: | Reconstitute in 50 µl or 200 µl deionized water, depending on the sample size. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | mGluR1 and mGluR5 receptors signal through Gq/G11 that activates phospholipase C and ultimately produces an increase in inositol triphosphate and cytosolic Ca2+. More downstream signaling pathways include activation of PKC and modulation of Ca2+ and K+ ion channels. Activation of signaling pathways independent of G proteins has also been reported. mGluR1 is predominantly expressed in nervous tissue although expression in several non-neural cell types has also been described. In the brain it is highly expressed in the hippocampus, cerebellum, olfactory bulb and thalamus. |
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Regulatory Status: | RUO - Research Use Only |
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Indirect flow cytometry analysis in live intact Jurkat cells:
Black: Unstained cells + goat-anti-rabbit-FITC.
Green: Cells + Anti-mGluR1 (extracellular) antibody (Cat# BML-SA610), (10 µg) + goat-anti-rabbit-FITC.
Immunoprecipitation of rat brain lysate:
1. Cell lysate + protein A beads + Anti-mGluR1 (extracellular) antibody (Prod. No. BML-SA610).
2. Cell lysate + protein A beads + pre-immune rabbit serum.
3. Cell lysate.
Black arrow indicates the mGluR1 protein (glycosylated form) while the red arrow shows the IgG heavy chain. Immunoblot was performed with the Anti-mGluR1 (extracellular) antibody.
Expression of mGluR1 in rat C6 glioma cells: Immunocytochemical staining of live intact rat C6 glioma cells using Anti-mGluR1 (extracellular) antibody (Cat# BML-SA610), (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were stained with Hoechst 33342 (blue).
Western blot analysis of rat brain lysate: 1. Anti-mGluR1 (extracellular) antibody (Cat# BML-SA610), (1:200). 2. Anti-mGluR1 (extracellular) antibody, preincubated with the control peptide antigen.
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General Literature References
Metabotropic glutamate receptors (mGluR5) activate transient receptor potential canonical channels to improve the regularity of the respiratory rhythm generated by the pre-Bötzinger complex in mice: F. Ben-Mabrouk, et al.; Eur. J. Neurosci.
35, 1725 (2012),
Abstract;
Deleterious Effects of Amyloid β Oligomers Acting as an Extracellular Scaffold for mGluR5: M. Renner, et al.; Neuron
66, 739 (2010),
Abstract;
Disassembly of shank and homer synaptic clusters is driven by soluble beta-amyloid(1-40) through divergent NMDAR-dependent signalling pathways: F. Roselli, et al.; PLoS One
4, e6011 (2009),
Abstract;
Metabotropic glutamate receptors: F. Ferraguti, et al.; Cell Tissue Res.
326, 483 (2006),
Abstract;
Regulation of metabotropic glutamate receptor signaling, desensitization and endocytosis: G.K. Dhami, et al.; Pharmacol. Ther.
111, 260 (2006),
Abstract;
Ionotropic and metabotropic glutamate receptor structure and pharmacology: J.N. Kew, et al.; Psychopharmacology (Berl)
179, 4 (2005),
Abstract;