| Alternative Name: | ET-A |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 413-426 of rat ET-A. |
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| UniProt ID: | P26684 |
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| Species reactivity: | Rat
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| Applications: | IHC, WB
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| Blocking Peptide: | Included: ET-A Control Peptide (40µg). Supplied lyophilized.
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| Purity Detail: | Affinity purified IgG. |
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| Formulation: | Lyophilized from PBS, pH 7.4, 1% BSA and 0.05% sodium azide. |
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| Reconstitution: | To lyophilized antibody, add 50µl or 200µl (depending on which size was purchased) of deionized water. Further dilutions should be made using a carrier protein such as 1% BSA. To lyophilized control peptide, add 100µl of distilled water. |
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| Use/Stability: | Lyophilized antibody and lyophilized peptide can be stored intact at room temperature for several weeks. For longer periods they should be stored at -20°C. After reconstitution, the antibody solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below. Centrifuge all antibody preparations before use (10,000 X g for 5 minutes). After reconstitution, control peptide should be stored at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | PREADSORPTION CONTROL: 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.05% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments. |
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| Regulatory Status: | RUO - Research Use Only |
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