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14-3-3ε (CT) polyclonal antibody



BML-SA475-0100	100 µl	346.00 USD
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Replaces Prod. #: ALX-215-022

Product Details

Alternative Name:	KCIP-1, Protein kinase C inhibitor protein 1, 14-3-3E

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to aa 239-255 (CG²³⁹EEQNKEALQDVEDENQ²⁵⁵) of 14-3-3ε.

UniProt ID:	P62258 (human)

Species reactivity:	Human, Mouse, Rat Bovine, Chicken, Rabbit, Sheep

Specificity:	Specific for the ε isoform.

Applications:	ELISA, IHC, WB

Recommended Dilutions/Conditions:	Western Blot (HRP/ECL detection, 1:3,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Blocking Peptide:	For Blocking peptide see Prod. No. BML-SP475.

Formulation:	Liquid. Neat serum. Contains no preservatives.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Blue Ice

Long Term Storage:	-20°C

Regulatory Status:	RUO - Research Use Only

14-3-3ε (CT) polyclonal antibody Western blot

Figure: Western blot analysis of cell lysates using 14-3-3 ε, pAb (Prod. No. BML-SA475). Antibody dilution 1:3’000, blot was developed by HRP/ECL.1. HeLa S100 (cytosol)2. HeLa (whole cell)3. Jurkat 4. MDCK 5. A4316. MDF-77. rat brain 8. PC12

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Product Literature References

Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005), Abstract; Full Text

Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003), Abstract;

Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002), Abstract;

Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002), Abstract;

Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999), Abstract;

14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997), Abstract; Full Text

Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996), Abstract; Full Text

Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995), Abstract;

Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995), Abstract;

Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994), Abstract;

Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994), Abstract;

Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993), Abstract;