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α-Synuclein polyclonal antibody

BML-SA3400-0025 25 µl 137.00 USD
BML-SA3400-0100 100 µl 444.00 USD
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Product Details

Alternative Name:NACP, Non-A β component of senile plaques
Immunogen:Synthetic peptide corresponding to [Cys] + aa 117-131 of human α-synuclein conjugated to KLH. The sequence is conserved in rat α-synuclein except for two substitutions, namely Asp121Ser and Asn122Ser.
UniProt ID:P37840
GenBank ID:2136215
Source:Purified from rabbit serum.
Species reactivity:Human, Rat
Specificity:This antibody has been shown to react strongly with human brain-derived α-synuclein. The antibody also reacts with rat brain-derived α-synuclein but reactivity appears substantially less than with human.
Crossreactivity:Does not cross-react with mouse α-synuclein.
Applications:IHC, WB
Recommended Dilutions/Conditions:Western blot (1:1,000-1:20,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Western blot: The antibody has been characterized by single dimension SDS-PAGE using recombinant α- and β-synucleins as well as rat, mouse and human brain lysates with and without peptide blockade. Studies with the recombinant proteins demonstrated the absolute specificity of the antibody for α-synuclein. The antibody gives a strong band at a relative molecular weight of approximately 15kDa with human brain lysate which is wholly abolished by preincubation with the immunizing peptide (Prod. No. BML-SP9340).

Immunohistochemistry: The antibody has been tested on de-waxed tissue sections of human brain fixed in neutral buffered formaldehyde. A standard protocol should be followed.
Blocking Peptide:For Blocking peptide see Prod. No. BML-SP9340.
Purity Detail:Purified by caprylic acid and ammonium sulphate precipitation.
Formulation:Liquid. In PBS, pH 7.4, containing 10mM sodium azide.
Use/Stability:Vial contents, Storage and Use: Vial contains an immunoglobulin preparation, partially purified by chromatography from whole serum, suspended in phosphate-buffered saline (pH 7.4) containing 0.01M sodium azide. Store unopened vial at -20°C until required for use. AVOID REPEATED FREEZE-THAW CYCLES. Aliquot undiluted antibody into smaller volumes (not less than 10μL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:α-Synuclein accumulates in Lewy bodies, which are intraneuronal cytoplasmic inclusions present in the brains of sporadic Parkinson's disease patients, and is implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders. α-Synuclein appears to associate with other proteins that aggregate and is found in β-amyloid plaques and neuritic tangles in Alzheimer's disease.
Regulatory Status:RUO - Research Use Only

Product Literature References

Small soluble α-synuclein aggregates are the toxic species in Parkinson’s disease: D. Emin, et al.; Nat. Commun. 13, 5512 (2022), Abstract;
The histone deacetylase inhibitor M344 as a multifaceted therapy for pancreatic cancer: S.M. Knoche, et al.; PLoS One 17, e0273518 (2022), Abstract;
A53T Human α-Synuclein Overexpression in Transgenic Mice Induces Pervasive Mitochondria Macroautophagy Defects Preceding Dopamine Neuron Degeneration: L. Chen, et al.; J. Neurosci. 35, 890 (2015), Application(s): Immunofluorescence, Postembedding Immunogold Electron Microscopy, Western Blotting, , Abstract;

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