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PARP polyclonal antibody

BML-SA253-0025 25 µl 170.00 USD
BML-SA253-0100 100 µl 414.00 USD
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Replaces Prod. #: ALX-210-222

Product Details

Alternative Name:Poly(ADP-ribose) polymerase, PARP-1
Immunogen:Synthetic peptide corresponding to aa 509-524 of bovine PARP automodification domain.
UniProt ID:P18493
Source:Whole rabbit serum.
Species reactivity:Human, Mouse, Rat
Applications:ELISA, WB
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric). Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application
Application Notes:Detects a band of ~116kDa by Western blot.
Formulation:Liquid. Neat serum containing 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Dry Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
PARP polyclonal antibody Western blot
Western Blot Analysis of the Poly(ADP-ribose) polymerase, pAb (BML-SA253). Lane 1: Molecular Weight Marker, Lane 2: Human HL60 Cell Extract (BML-SW101).
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PARP polyclonal antibody Western blot

Product Literature References

Structure-Activity Relationship of Hydroxycinnamic Acid Derivatives for Cooperating with Carnosic Acid and Calcitriol in Acute Myeloid Leukemia Cells: A. Trachtenberg, et al.; Biomedicines 9, 1517 (2021), Abstract;
Synergistic cytotoxicity of methyl 4-hydroxycinnamate and carnosic acid to acute myeloid leukemia cells via calcium-dependent apoptosis induction: A. Trachtenberg, et al.; Front. Pharmacol. 10, 507 (2019), Application(s): Western-blot, Abstract; Full Text
Cancer-selective cytotoxic Ca2+ overload in acute myeloid leukemia cells and attenuation of disease progression in mice by synergistically acting: S. Pesakhov, et al.; Oncotarget 7, 31847 (2016), Application(s): Western-blot, Abstract; Full Text
Gestational diabetes mellitus alters apoptotic and inflammatory gene expression of trophobasts from human term placenta: T.R. Magee, et al.; J. Diabetes Complications 28, 448 (2014), Application(s): Western-blot, Abstract; Full Text
Increased PARP-1 association with DNA in alkylation damaged, PARP-inhibited mouse fibroblasts: P.S. Kedar, et al.; Mol. Cancer Res. 10, 360 (2012), Application(s): Western-blot, Abstract; Full Text

General Literature References

Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase.: P.J. Duriez; Biochim. Biophys Acta 1334, 65 (1997), Abstract;
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE.: Y.A. Lazebnik et al.; Nature 371, 346 (1994), Abstract;
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study.: D. Lamarre et al.; Biochim. Biophys. Acta 950, 147 (1988), Abstract;

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The PARP Family & ADP Ribosylation
The PARP Family & ADP Ribosylation
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