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PARP-1 monoclonal antibody (C-2-10) (affinity purified)

BML-SA249-0050 50 µg 428.00 USD
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Replaces Prod. #: ALX-804-210

Product Details

Alternative Name:Poly(ADP-ribose) polymerase-1
Immunogen:Purified calf thymus poly(ADP-ribose) polymerase (PARP).
UniProt ID:P18493
Source:Purified from ascites.
Species reactivity:Human, Mouse, Rat
Bovine, Hamster, Monkey
Specificity:Recognizes an epitope in the C-terminal part of the DNA binding domain of PARP.
Crossreactivity:Does not cross-react with chicken PARP.
Applications:ELISA, ICC, WB
Recommended Dilutions/Conditions:Western Blot (1µg/ml, alkaline phosphatase)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects bands ~116kDa (intact PARP) and ~85kDa (apoptosis-induced cleavage fragment) by Western blot.
Purity Detail:Affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Dry Ice
Long Term Storage:-20°C
Regulatory Status:RUO - Research Use Only
PARP-1 monoclonal antibody (C-2-10) (affinity purified) Western blot
Western blot analysis of whole cell extracts from (1) human HL60 leukemia cells (Prod. No. BML-SW101) and (2) HL60 cells induced to undergo apoptosis using etoposide (Prod. No. BML-SW102). The nitrocellulose membrane was probed with purified clone C-2-10 mouse monoclonal anti-PARP (Prod. No. BML-SA249) at 1 µg/ml. Secondary antibody was GAM-AP (1/2000) and the nitrocellulose membrane was developed with BCIP/NBT. Arrows correspond to 116kDa and 85kDa.
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PARP-1 monoclonal antibody (C-2-10) (affinity purified) Western blot

Product Literature References

Expression of BRCA1, BRCA2, RAD51, and other DSB repair factors is regulated by CRL4WDR70: Z. Mirman, et al.; DNA Repair (Amst.) 113, 103320 (2022), Abstract;
New Insights into the Significance of PARP-1 Activation: Flow Cytometric Detection of Poly(ADP-Ribose) as a Marker of Bovine Intramammary Infection: G.D. Matteis, et al.; Cells 10, 599 (2021), Abstract;
Combinatory Treatment of Canavanine and Arginine Deprivation Efficiently Targets Human Glioblastoma Cells via Pleiotropic Mechanisms: O. Karatsai, et al.; Cells 9, 2217 (2020), Abstract;
Autophagy requires poly(adp-ribosyl)ation-dependent AMPK nuclear export: J.M. Rodríguez-Vargas, et al.; Cell Death Differ. 23, 2007 (2016), Application(s): Western blot, MCF7 GFP-LC3 cells, Abstract;
PRIMA-1(MET) induces death in soft-tissue sarcomas cell independent of p53: T. Grellety, et al.; BMC Cancer 15, 684 (2015), Application(s): Immunoblot analysis, Abstract;
Alzheimer's disease-associated polymorphisms in human OGG1 alter catalytic activity and sensitize cells to DNA damage: K.D. Jacob, et al.; Free Radic. Biol. Med. 63, 115 (2013), Application(s): Western blot, Abstract;
Regulation of poly(ADP-ribose) polymerase-1 functions by leukocyte elastase inhibitor/LEI-derived DNase II during caspase-independent apoptosis: C. Leprête, et al.; Int. J. Biochem. Cell Biol. 41, 1046 (2009), Abstract;
CCCTC-binding Factor Activates PARP-1 Affecting DNA Methylation Machinery: T. Guastafierro, et al.; J. Biol. Chem. 283, 21873 (2008), Abstract; Full Text
Nuclear poly(ADP-ribose) polymerase-1 rapidly triggers mitochondrial dysfunction: G. Cipriani, et al.; J. Biol. Chem. 280, 17227 (2005), Abstract; Full Text
Inactivation of p21WAF1 sensitizes cells to apoptosis via an increase of both p14ARF and p53 levels and an alteration of the Bax/Bcl-2 ratio: D. Javelaud & F. Besançon; J. Biol. Chem. 277, 37949 (2002), Abstract; Full Text
Characterization of the necrotic cleavage of poly(ADP-ribose) polymerase (PARP-1): implication of lysosomal proteases: S. Gobeil, et al.; Cell Death Differ. 8, 588 (2001), Abstract;
Selective loss of poly(ADP-ribose) and the 85-kDa fragment of poly(ADP- ribose) polymerase in nucleoli during alkylation-induced apoptosis of HeLa cells: R. Alvarez-Gonzalez, et al.; J. Biol. Chem. 274, 32122 (1999), Abstract; Full Text
Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem. 272, 1965 (1997), Abstract;
Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase: P.J. Duriez, et al.; Biochim. Biophys. Acta 1334, 65 (1997), Abstract;
Different cleavage pattern for poly(ADP-ribose) polymerase during necrosis and apoptosis in HL-60 cells: G.M. Shah, et al.; Biochem. Biophys. Res. Commun. 229, 838 (1996), Abstract;
FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis: A.M. Chinnaiyan, et al.; J. Biol. Chem. 271, 4961 (1996), Abstract; Full Text
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS 92, 9042 (1995), Abstract; Full Text
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994), Abstract;
Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis: S.H. Kaufmann, et al.; Cancer Res. 53, 3976 (1993), Abstract;
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study: D. Lamarre, et al.; Biochim. Biophys. Acta 950, 147 (1988), Abstract;

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