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nNOS polyclonal antibody

BML-SA227-0025 25 µg 154.00 USD
BML-SA227-0100 100 µg 515.00 USD
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Product Details

Alternative Name:Nitric oxide synthase (neuronal), NOS I
Immunogen:Synthetic peptide corresponding to aa 1414-1434 (CRSESIAFIEESKKDTDEVFSS) of human brain NOS (nNOS; NOS1).
UniProt ID:P29475
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Applications:ELISA, ICC, IF, WB
Recommended Dilutions/Conditions:Western Blot (1-5 µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Protein A-affinity purified.
Formulation:Liquid. In PBS containing 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.
Regulatory Status:RUO - Research Use Only
nNOS polyclonal antibody Western blot
Western blot analysis of nNOS, pAb (Prod. No. BML-SA227) using whole cell extracts from (1) rat brain (BML-SW103), (2) mouse brain (Prod. No. BML-SW104), and (3,4) 10 ng recombinant rat bNOS (Prod. No. BML-SW109). In lane (4) the antibody was preincubated with Prod. No. BML-SP227 blocking control peptide. The nitrocellulose membrane was probed with Prod. No. BML-SA227 at 5 μg/ml. The secondary antibody was GAR-AP diluted 1/2000 and the blot was developed with BCIP/NBT.
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nNOS polyclonal antibody Western blot

Product Literature References

Renal collecting duct NOS1 maintains fluid-electrolyte homeostasis and blood pressure: K.A. Hyndman, et al.; Hypertension 2, 91 (2017), Abstract; Full Text
Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia: H. Nagai, et al.; PLoS One 10, e0131923 (2015), Application(s): Immunocytochemistry, Western Blot, Abstract; Full Text

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