Product Details
| Alternative Name: | iNOS, NOS II |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 1131-1141 of mouse macrophage NOS. |
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| UniProt ID: | P29477 |
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| Gene/Protein Identifier: | NM_010927 (RefSeq) |
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| Species reactivity: | Human, Mouse, Rat
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| Crossreactivity: | Does not cross-react with eNOS or nNOS. |
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| Applications: | ICC, IHC, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:500)
Immunohistochemistry (1:500)
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~130kDa by Western blot. |
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| Blocking Peptide: | For Blocking peptide see Prod. No. BML-SP200.
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| Formulation: | Liquid. In PBS containing 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of lysate from mouse macrophage RAW 264.7 cell line, which was induced with IFNγ and LPS. The nitrocellulose membrane was probed with Anti-iNOS [Prod. no. BML-SA200] at 1/1000 dilution. Blot was developed with “Vectastain ABC Kit” and BCIP/NBT. Arrow points to MW ~130kDa.
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Product Literature References
NecroX-7 reduces necrotic core formation in atherosclerotic plaques of Apoe knockout mice: M.O.J. Grootaert, et al.; Atherosclerosis
252, 166 (2016),
Abstract;
Photobiomodulation rescues the cochlea from noise-induced hearing loss via upregulating nuclear factor κB expression in rats: A. Tamura, et al.; Brain Res.
1646, 467 (2016),
Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci.
161, 37 (2016),
Application(s): Western blot; evaluation of the role of VD2 on anti-inflammation,
Abstract;
Low-level laser therapy for prevention of noise-induced hearing loss in rats: A. Tamura, et al.; Neurosci. Lett.
595, 81 (2015),
Application(s): Immunohistochemistry,
Abstract;
Loss of methylation in CpG sites in the NF-κB enhancer elements of inducible nitric oxide synthase is responsible for gene induction in human articular chondrocytes: M.C. De Andres, et al.; Arthritis Rheum.
65, 732 (2013),
Abstract;
Full Text
Expression of inducible nitric oxide synthase and cyclooxygenase-2 in ovarian cancer: correlation with clinical outcome: M.R. Raspollini, et al.; Gynecol. Oncol.
92, 806 (2004),
Abstract;
Altered expression of inducible nitric oxide synthase (iNOS) in the cochlea: X. Shi, et al.; Hear. Res.
177, 43 (2003),
Abstract;
Effect of SNI-2011 on amylase secretion from parotid tissue in rats and in neuronal nitric oxide synthase knockout mice: Z. Yuan, et al.; Eur. J. Pharmacol.
464, 197 (2003),
Abstract;
Evidence for a functional nitric oxide synthase system in brown adipocyte nucleus: A. Giordano, et al.; FEBS Lett.
514, 135 (2002),
Abstract;
General Literature References
Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme: C.J. Lowenstein, et al.; PNAS
89, 6711 (1992),
Abstract;
Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line: C.R. Lyons, et al.; J. Biol. Chem.
267, 6370 (1992),
Abstract;
D. Bredt, et al.; Nature 351, 71 (1991),
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