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Nitric oxide synthase (inducible) polyclonal antibody

BML-SA200-0025 25 µl 199.00 USD
BML-SA200-0100 100 µl 611.00 USD
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Product Details

Alternative Name:iNOS, NOS II
Immunogen:Synthetic peptide corresponding to aa 1131-1141 of mouse macrophage NOS.
UniProt ID:P29477
Gene/Protein Identifier:NM_010927 (RefSeq)
Species reactivity:Human, Mouse, Rat
Crossreactivity:Does not cross-react with eNOS or nNOS.
Applications:ICC, IHC, WB
Recommended Dilutions/Conditions:Immunocytochemistry (1:500)
Immunohistochemistry (1:500)
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~130kDa by Western blot.
Blocking Peptide:For Blocking peptide see Prod. No. BML-SP200.
Formulation:Liquid. In PBS containing 0.05% sodium azide.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Shipping:Dry Ice
Long Term Storage:-80°C
Regulatory Status:RUO - Research Use Only
Nitric oxide synthase (inducible) polyclonal antibody Western blot
Western blot analysis of lysate from mouse macrophage RAW 264.7 cell line, which was induced with IFNγ and LPS. The nitrocellulose membrane was probed with Anti-iNOS [Prod. no. BML-SA200] at 1/1000 dilution. Blot was developed with “Vectastain ABC Kit” and BCIP/NBT. Arrow points to MW ~130kDa.
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Nitric oxide synthase (inducible) polyclonal antibody Western blot

Product Literature References

NecroX-7 reduces necrotic core formation in atherosclerotic plaques of Apoe knockout mice: M.O.J. Grootaert, et al.; Atherosclerosis 252, 166 (2016), Abstract;
Photobiomodulation rescues the cochlea from noise-induced hearing loss via upregulating nuclear factor κB expression in rats: A. Tamura, et al.; Brain Res. 1646, 467 (2016), Abstract;
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Western blot; evaluation of the role of VD2 on anti-inflammation, Abstract;
Low-level laser therapy for prevention of noise-induced hearing loss in rats: A. Tamura, et al.; Neurosci. Lett. 595, 81 (2015), Application(s): Immunohistochemistry, Abstract;
Loss of methylation in CpG sites in the NF-κB enhancer elements of inducible nitric oxide synthase is responsible for gene induction in human articular chondrocytes: M.C. De Andres, et al.; Arthritis Rheum. 65, 732 (2013), Abstract; Full Text
Expression of inducible nitric oxide synthase and cyclooxygenase-2 in ovarian cancer: correlation with clinical outcome: M.R. Raspollini, et al.; Gynecol. Oncol. 92, 806 (2004), Abstract;
Altered expression of inducible nitric oxide synthase (iNOS) in the cochlea: X. Shi, et al.; Hear. Res. 177, 43 (2003), Abstract;
Effect of SNI-2011 on amylase secretion from parotid tissue in rats and in neuronal nitric oxide synthase knockout mice: Z. Yuan, et al.; Eur. J. Pharmacol. 464, 197 (2003), Abstract;
Evidence for a functional nitric oxide synthase system in brown adipocyte nucleus: A. Giordano, et al.; FEBS Lett. 514, 135 (2002), Abstract;

General Literature References

Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme: C.J. Lowenstein, et al.; PNAS 89, 6711 (1992), Abstract;
Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line: C.R. Lyons, et al.; J. Biol. Chem. 267, 6370 (1992), Abstract;
D. Bredt, et al.; Nature 351, 71 (1991),

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