Product Details
| Alternative Name: | Proteasome subunit β type-5, Macropain ε chain, Proteasome chain 6, Proteasome subunit MB1, Proteasome subunit X |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 194-207 of human proteasome 20S (β5 subunit). |
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| UniProt ID: | P28074 |
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| Gene/Protein Identifier: | PSMB5 (gene name) |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human
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| Specificity: | Recognizes human proteasome 20S β5 subunit. |
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| Applications: | IHC, WB
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| Formulation: | Liquid. In PBS containing 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Healthspan improvement and anti-aggregation effects induced by a marine-derived structural proteasome activator: M.A. Vasilopoulou, et al.; Redox Biol.
56, 10262 (2022),
Abstract;
High Immunoproteasome Activity and sXBP1 in Pediatric Precursor B-ALL Predicts Sensitivity towards Proteasome Inhibitors: L. Besse, et al.; Cells
10, 2853 (2021),
Abstract;
Immobilized Antibodies on Mercaptophenylboronic Acid Monolayers for Dual-Strategy Detection of 20S Proteasome: M.M. Barsan, et al.; Sensors
21, 2702 (2021),
Abstract;
Full Text
An SOD1 deficiency aggravates proteasome inhibitor bortezomib-induced testicular damage in mice: T. Homma & J. Fujii; Biochim. Biophys. Acta Gen. Subj.
1863, 1108 (2019),
Application(s): Mouse cryosectioned tissue,
Abstract;
Cholesterol Metabolism Is a Druggable Axis that Independently Regulates Tau and Amyloid-β in iPSC-Derived Alzheimer's Disease Neurons: R. van der Kant, et al.; Cell Stem Cell
24, 363 (2019),
Application(s): Western blot,
Abstract;
Full Text
Novel cell line models to study mechanisms and overcoming strategies of proteasome inhibitor resistance in multiple myeloma: D. Brunnert, et al.; Biochim. Biophys. Acta Mol. Basis Dis.
1865, 1666 (2019),
Abstract;
Involvement of the ubiquitin-proteasome system in the expression of extracellular matrix genes in retinal pigment epithelial cells: J.E. Ramos de Carvalho, et al.; Biochem. Biophys. Rep.
13, 83 (2018),
Abstract;
Modulation of the Proteasome Pathway by Nano-Curcumin and Curcumin in Retinal Pigment Epithelial Cells: J.E. Ramos de Carvalho, et al.; Ophthalmic Res.
59, 98 (2018),
Abstract;
Potential involvement of ubiquitin-proteasome system dysfunction associated with oxidative stress in the pathogenesis of sickle cell disease: P. Warang, et al.; Br. J. Haematol.
182, 559 (2018),
Abstract;
A mammalian nervous-system-specific plasma membrane proteasome complex that modulates neuronal function: K.V. Ramachandran, et al.; Nat. Struct. Mol. Biol.
24, 419 (2017),
Abstract;
Full Text
A strategy for dual inhibition of the proteasome and fatty acid synthase with belactosin C-orlistat hybrids: M. Zhu, et al.; Bioorg. Med. Chem.
25, 2901 (2017),
Abstract;
Full Text
A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens: A.Y. Chang, et al.; J. Clin. Invest.
127, 2705 (2017),
Abstract;
Full Text
Detection of active proteasome structures in brain extracts: proteasome features of August rat brain with violations in monoamine metabolism: P.A. Erokhov, et al.; Oncotarget
8, 70695 (2017),
Abstract;
Full Text
Low Erythrocyte Levels of Proteasome and Acyl-Peptide Hydrolase (APEH) Activities in Alzheimer's Disease: A Sign of Defective Proteostasis?: G. Palmieri, et al.; J. Alzheimers Dis.
60, 1097 (2017),
Abstract;
Proteasome Activation by Small Molecules: Y. Leestemaker, et al.; Cell Chem. Biol.
24, 725 (2017),
Abstract;
Proteasome activation enhances stemness and lifespan of human mesenchymal stem cells: M. Kapetanou, et al.; Free Radic. Biol. Med.
103, 226 (2017),
Abstract;
The proteasome maturation protein POMP increases proteasome assembly and activity in psoriatic lesional skin: B.A. Zieba, et al.; J. Dermatol. Sci.
88, 10 (2017),
Abstract;
Circadian modulation of proteasome activity and accumulation of oxidized protein in human embryonic kidney HEK 293 cells and primary dermal fibroblasts: A. Desvergne, et al.; Free Radic. Biol. Med.
94, 195 (2016),
Application(s): Western blot,
Abstract;
Interferon-dependent immunoproteasome activity during mouse adenovirus type 1 infection: M.K. McCarthy, et al.; Virology
498, 57 (2016),
Abstract;
Full Text
Efficacy of panobinostat and marizomib in acute myeloid leukemia and bortezomib-resistant models: F.F. Corrales-Medina, et al.; Leuk. Res.
39, 371 (2015),
Application(s): Western Blotting,
Abstract;
Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux: L.M. Bollinger, et al.; Obesity (Silver Spring)
23, 1185 (2015),
Application(s): Western Blot,
Abstract;
Full Text
SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes: T. Homma, et al.; Arch. Biochem. Biophys.
583, 65 (2015),
Application(s): Immunoblotting,
Abstract;
The GST-BHMT assay reveals a distinct mechanism underlying proteasome inhibition-induced macroautophagy in mammalian cells: Y.N. Rui, et al.; Autophagy
11, 812 (2015),
Abstract;
Full Text
Ubiquitin-independent proteosomal degradation of myelin basic protein contributes to development of neurodegenerative autoimmunity: A. Belogurov Jr, et al.; FASEB J.
29, 1901 (2015),
Abstract;
Full Text
The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a: A. Sparks, et al.; Oncogene
33, 4685 (2014),
Application(s): WB using human cell lysates,
Abstract;
Full Text
The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget
5, 6252 (2014),
Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay,
Abstract;
Full Text
Negative regulation of 26S proteasome stability via calpain-mediated cleavage of Rpn10 subunit upon mitochondrial dysfunction in neurons: Q. Huang, et al.; J. Biol. Chem.
288, 12161 (2013),
Abstract;
Proteasome inhibitors block DNA repair and radiosensitize non-small cell lung cancer: K.R. Cron, et al.; PLoS One
8, e73710 (2013),
Application(s): Western blot,
Abstract;
Full Text
Proteasome inhibitors protect the steatotic and non-steatotic liver graft against cold ischemia reperfusion injury: M.A. Zaouali, et al.; Exp. Mol. Pathol.
94, 352 (2013),
Application(s): Western blot,
Abstract;
RedOx status, proteasome and APEH: insights into anticancer mechanisms of t10, c12-conjugated linoleic acid isomer on A375 melanoma cells: P. Bergamo, et al.; PLoS One
8, e80900 (2013),
Application(s): Western blot,
Abstract;
Full Text
A putative role for the immunoproteasome in the maintenance of pluripotency in human embryonic stem cells: S.P. Atkinson, et al.; Stem Cells
30, 1373 (2012),
Application(s): Western blot,
Abstract;
Full Text
Bortezomib resistance in a myeloma cell line is associated to PSMβ5 overexpression and polyploidy: P. Balsas, et al.; Leuk. Res.
36, 212 (2012),
Application(s): Western blot,
Abstract;
Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62: N. Myeku, et al.; J. Biol. Chem.
286, 22426 (2011),
Application(s): Western blot,
Abstract;
Full Text
TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions: Z. Lukic, et al.; Retrovirology
8, 93 (2011),
Abstract;
Full Text
