Product Details
| Clone: | UBCJ2 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Poly-ubiquitinylated lysozyme. |
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| UniProt ID: | P0CG47 (UBB), P0CG48 (UBC) |
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| Source: | Purified from tissue culture supernatant. |
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| Species reactivity: | Species independent
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| Specificity: | Recognizes mono- and polyubiquitinylated protein conjugates in a wide range of species. |
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| Applications: | ELISA, Flow Cytometry, ICC, WB
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| Recommended Dilutions/Conditions: | ELISA (1:1000)
Immunocytochemistry (1:250)
Flow cytometry (1:100)
Western Blot (1:1000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.09% sodium azide. |
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| Handling: | Store unopened vial at -20°C. Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ-ABS840F): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250 dilution. Panel A: isotype control (Prod. No. ADI-SAB-600FI); Panel B: untreated; Panel C: proteasome inhibitor-treated HeLa cells. Left: 20X and Right: 63X amplified.
Flow cytometry analysis of 106 Jurkat cells stained using Mono- and polyubiquitinylated conjugates mAb (UBCJ2) (FITC conjugate), (Prod. No. ENZ-ABS840F), at a concentration of 10µg/ml, side-by-side comparison to Mono- and polyubiquitinylated conjugates mAb (FK2) (fluorescein labeled), (Prod. No. BML-PW1210).
Western Blot analysis of ENZ-ABS840HRP detecting ubiquitin chains: Lane 1: MW Marker, Lane 2: K48-linked, Lane 3: K63-linked.
Western Blot analysis of ubiquitinylated conjugates in whole cell lysates using the Mono- and polyubiquitinylated conjugates mAb (UBCJ2) (Prod. No. ENZ-ABS840). HeLa cells were treated for 12 hours with DMSO (vehicle, lane 1) or proteasome inhibitor MG-132 at 25 µM (lane 2). Membrane was incubated O.N. (4°C) with 1 µg/mL of mAb clone UBCJ2. Detection was done with a goat anti-mouse secondary antibody. Ponceau-S staining was performed as loading control (bottom panel). Image is a courtesy of Dr Jacob Seeler, Nuclear Organization and Oncogenesis Unit, Pasteur Institute, Paris (FR).
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