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Proteasome 20S β5i subunit (human) polyclonal antibody

BML-PW8355-0025 25 µl 158.00 USD
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Replaces Prod. #: ALX-210-326

Product Details

Alternative Name:Proteasome subunit β type-8, LMP7, RING10 protein, Macropain subunit C13
Immunogen:Synthetic peptide corresponding to aa 262-276 (T262DVSDLLHQYREANQ276) of human proteasome 20S (β5i subunit).
UniProt ID:P28062
Gene/Protein Identifier:PSMB8 (gene name), 5696 (Entrez Gene ID)
Species reactivity:Human
Specificity:Recognizes the β5i subunit of the 20S proteasome.
Applications:IHC, WB
Application Notes:Detects bands of ~25kDa (mature proteasome 20S β5i subunit) and of ~29kDa (precursor) by Western blot.
Formulation:Liquid. Antiserum containing 10mM sodium azide.
Use/Stability:Dilute with PBS, pH 7.2-7.4 containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C (do not freeze) and use within 1 month.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles.  The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome.  Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle.  Each subunit is located in a unique position within the α- or β-rings. Lmp2, Lmp7 and MECL are interferon gamma-inducible catalytic subunits of the 20S proteasome which may replace the constitutive catalytic subunits, delta, X and Z respectively, during proteasome biogenesis.  Lmp2 and Lmp7 alter the cleavage site specificity of the 20S proteasome and are required for the efficient generation of T cell epitopes from a number of viral proteins and for optimal MHC class I cell surface expression.
Technical Info/Product Notes:Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.
Regulatory Status:RUO - Research Use Only
Proteasome 20S β5i subunit (human) polyclonal antibody Western blot
Figure: Western blot analysis of HeLa cell lysate with and without prior stimulation with interferon γ using Proteasome 20S beta5i subunit (human), pAb (Prod. No. BML-PW8355).Method: SDS-PAGE was followed by blotting onto PVDF and probing with antibody BML-PW8355 and others. Antibody dilution 1:10’000.
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Proteasome 20S β5i subunit (human) polyclonal antibody Western blot

Product Literature References

High Immunoproteasome Activity and sXBP1 in Pediatric Precursor B-ALL Predicts Sensitivity towards Proteasome Inhibitors: L. Besse, et al.; Cells 10, 2853 (2021), Abstract;
Novel cell line models to study mechanisms and overcoming strategies of proteasome inhibitor resistance in multiple myeloma: D. Brunnert, et al.; Biochim. Biophys. Acta Mol. Basis Dis. 1865, 1666 (2019), Abstract;
Detection of active proteasome structures in brain extracts: proteasome features of August rat brain with violations in monoamine metabolism: P.A. Erokhov, et al.; Oncotarget 8, 70695 (2017), Abstract; Full Text
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine 2, 642 (2015), Application(s): Western Blot, Abstract;
Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE): D.S. Pitcher, et al.; Biochim. Biophys. Acta 1844, 2222 (2014), Abstract;
TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions: Z. Lukic, et al.; Retrovirology 8, 93 (2011), Abstract; Full Text

General Literature References

Dendritic cells up-regulate immunoproteasomes and the proteasome regulator PA28 during maturation: A. Macagno, et al.; Eur. J. Immunol. 29, 4037 (1999), Abstract;
Immunoproteasome assembly: cooperative incorporation of interferon gamma (IFN-gamma)-inducible subunits: T.A. Griffin, et al.; J. Exp. Med. 187, 97 (1998), Abstract; Full Text
A third interferon-gamma-induced subunit exchange in the 20S proteasome: M. Groettrup, et al.; Eur. J. Immunol. 26, 863 (1996), Abstract;

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