Product Specification
| Host: | Rabbit |
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| Immunogen: | Recombinant human COP9 signalosome (Csn1 subunit) (aa 230-500). |
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| UniProt ID: | Q13098 |
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| Species reactivity: | Human, Mouse
Frog, Porcine
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| Crossreactivity: | Does not cross-react with plant COP9 signalosome (Csn1 subunit). |
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| Applications: | IHC, IP, WB
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| Formulation: | Liquid. In PBS containing 50% glycerol and 10mM sodium azide. |
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| Shipping: | Shipped on Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The COP9 signalosome, once defined as a repressor complex of light activated development in Arabidopsis, has recently been found in humans and is probably present in most multicellular organisms. The COP9 signalosome is closely related to the lid sub-complex of the 26S proteasome in structural composition and probably shares a common evolutionary ancestor. A multifaceted role of the COP9 signalosome in cell-signalling processes is hinted at by its associated novel kinase activity, as well as the involvement of its subunits in regulating multiple cell-signalling pathways and cell-cycle progression. The molecular genetic studies in Arabidopsis suggest that the complex functions as part of a highly conserved regulatory network, whose physiological role in animals has yet to be determined. The COP9 signalosome, also known as the the COP9 complex and JAB1-containing signalosome, is a conserved nuclear protein complex found in plants and animals. It is composed of eight distinct subunits, designated S1 to S8 (very recently re-designated Csn1-Csn82) according to the molecular weight of the mammalian complex subunits. Subunit composition and the subunit sequences are substantially conserved between the mammalian and the plant complexes, implying that the complex has a conserved cellular function in higher eukaryotic organisms. Subunit 1 (Csn1) was previously identified as GPS1, which was isolated by its ability to rescue the lethality of a S. cerevisiae mutant deleted for the α-subunit of trimeric G protein. Transient expression of full length GPS1 in mammalian cells was shown to inhibit JNK1 and to repress JUN-dependent promoter activity. Csn1 shows homology to 19S proteasome regulatory subunit Rpn7 (S10a). |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
COP9 signalosome interacts ATP-dependently with p97/valosin-containing protein (VCP) and controls the ubiquitination status of proteins bound to p97/VCP: S. Cayli, et al.; J. Biol. Chem.
284, 34944 (2009),
Abstract;
Making sense of the COP9 signalosome. A regulatory protein complex conserved from Arabidopsis to human: N. Wei & X.W. Deng; Trends. Genet.
15, 98 (1999),
Abstract;
Characterization and purification of the mammalian COP9 complex, a conserved nuclear regulator initially identified as a repressor of photomorphogenesis in higher plants: N. Wei & X.W. Deng; Photochem. Photobiol.
68, 237 (1998),
Abstract;
The COP9 complex is conserved between plants and mammals and is related to the 26S proteasome regulatory complex: N. Wei, et al.; Curr. Biol.
8, 16 (1998),
Abstract;
